Abstract
All the nine common cytokines in this study (including NT-3, IGF-1, CNTF and TGF-α) bind noncovalently, yet with different specificities and to different degrees, with both normal α2-macroglobulin (α2M) and monoamine-modified α2M. The binding of NGF is by far the most efficient and is least affected by cationic proteins. The binding of NT-3 is slightly affected by cationic proteins but is completely blocked by NGF. The binding of TGF-α, TGF-β1, CNTF, and IL-6 is severely blocked by cationic proteins/NGF. We conclude that NGF and NT-3 appear to bind specifically in significant quantities to the same α2M sites; but the other cytokines by comparison bind minimally, and primarily or entirely use nonspecific molecular interactions in their binding to α2M.
Original language | English (US) |
---|---|
Pages (from-to) | 1255-1261 |
Number of pages | 7 |
Journal | Biochemical and biophysical research communications |
Volume | 193 |
Issue number | 3 |
DOIs | |
State | Published - Jun 30 1993 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology