Comparative Binding of Neurotrophins (NT-3, CNTF and NGF) and Various Cytokines to α2-Macroglobulin

Daniel J. Liebl, Peter H. Koo

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

All the nine common cytokines in this study (including NT-3, IGF-1, CNTF and TGF-α) bind noncovalently, yet with different specificities and to different degrees, with both normal α2-macroglobulin (α2M) and monoamine-modified α2M. The binding of NGF is by far the most efficient and is least affected by cationic proteins. The binding of NT-3 is slightly affected by cationic proteins but is completely blocked by NGF. The binding of TGF-α, TGF-β1, CNTF, and IL-6 is severely blocked by cationic proteins/NGF. We conclude that NGF and NT-3 appear to bind specifically in significant quantities to the same α2M sites; but the other cytokines by comparison bind minimally, and primarily or entirely use nonspecific molecular interactions in their binding to α2M.

Original languageEnglish (US)
Pages (from-to)1255-1261
Number of pages7
JournalBiochemical and biophysical research communications
Volume193
Issue number3
DOIs
StatePublished - Jun 30 1993
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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