Comparative binding of neurotrophins (NT-3, CNTF and NGF) and various cytokines to α2-macroglobulin

Daniel J Liebl, P. H. Koo

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

All the nine common cytokines in this study (including NT-3, IGF-1, CNTF and TGF-α) bind noncovalently, yet with different specificities and to different degrees, with both normal α2-macroglobulin (α2M) and monoamine-modified α2M. The binding of NGF is by far the most efficient and is least affected by cationic proteins. The binding of NT-3 is slightly affected by cationic proteins but is completely blocked by NGF. The binding of TGF-α, TGF-β1, CNTF, and IL-6 is severely blocked by cationic proteins/NGF. We conclude that NGF and NT-3 appear to bind specifically in significant quantities to the same α2M sites; but the other cytokines by comparison bind minimally, and primarily or entirely use nonspecific molecular interactions in their binding to α2M.

Original languageEnglish
Pages (from-to)1255-1261
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume193
Issue number3
DOIs
StatePublished - Jan 1 1993
Externally publishedYes

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Ciliary Neurotrophic Factor
Neurotrophin 3
Macroglobulins
Nerve Growth Factors
Nerve Growth Factor
Cytokines
Proteins
Molecular interactions
Insulin-Like Growth Factor I
Interleukin-6

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Comparative binding of neurotrophins (NT-3, CNTF and NGF) and various cytokines to α2-macroglobulin. / Liebl, Daniel J; Koo, P. H.

In: Biochemical and Biophysical Research Communications, Vol. 193, No. 3, 01.01.1993, p. 1255-1261.

Research output: Contribution to journalArticle

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