Comparative analysis of lymphocytes in lymph nodes and peripheral blood of patients with chronic fatigue syndrome

M. A. Fletcher, K. Maher, R. Patarca-Montero, N. Klimas

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Blood and lymph node samples were obtained from patients with chronic fatigue syndrome (CFS) who had volunteered to undergo a lymph node biopsy while participating in a phase 1 clinical trial of a novel immunomodulatory therapy. The surface marker phenotypes of the peripheral blood and lymph node samples were examined using four-color flow cytometry and compared to published proportions of cells in peripheral blood and lymph nodes from control individuals. While a greater proportion of T lymphocytes from both lymph nodes and peripheral blood of control subjects are immunologically "naive" (CD45RA+), the proportions of lymphocytes with a "memory" phenotype predominate in lymph nodes and peripheral blood of CFS patients. CFS has been proposed to be a disease of autoimmune etiology and in this respect it is interesting to note that decreased proportions of CD45RA+ T ("naive") cells are also seen in the peripheral blood of patients with autoimmune diseases.

Original languageEnglish (US)
Pages (from-to)65-75
Number of pages11
JournalJournal Of Chronic Fatigue Syndrome
Volume7
Issue number3
DOIs
StatePublished - Jan 1 2000

Keywords

  • Chronic fatigue syndrome
  • Four-color flow cytometry
  • Lymph node
  • Memory T cells
  • Naive T cells

ASJC Scopus subject areas

  • Neuropsychology and Physiological Psychology

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