Common pattern of two distinct types of colony-stimulating factor in human tissues and cultured cells

M. C. Wu, Adel A Yunis

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Conditioned media prepared from human lung, placenta, peripheral leukocytes, cultured human pancreatic carcinoma cells, and cultured cervical carcinoma cells exhibit a common pattern of two distinct colony-stimulating factors (CSF) separable by isoelectrofocusing. Type I CSF appears polydisperse on isoelectrofocusing, showing multiple peaks with an isoelectric point of 3.6-4.7 and mol wt 50,000. It has a much higher activity in mouse than in human bone marrow, and stimulates the formation of predominately macrophage colonies. Type II CSF has an isoelectric point of 5.7 and mol wt 27,000 and exhibits higher activity in human than in mouse marrow, yielding predominately granulocytic colonies.

Original languageEnglish (US)
Pages (from-to)772-775
Number of pages4
JournalJournal of Clinical Investigation
Volume65
Issue number3
StatePublished - 1980

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Colony-Stimulating Factors
Cultured Cells
Isoelectric Point
Bone Marrow
Conditioned Culture Medium
Human Activities
Placenta
Leukocytes
Macrophages
Carcinoma
Lung

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Common pattern of two distinct types of colony-stimulating factor in human tissues and cultured cells. / Wu, M. C.; Yunis, Adel A.

In: Journal of Clinical Investigation, Vol. 65, No. 3, 1980, p. 772-775.

Research output: Contribution to journalArticle

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