Commissureless endocytosis is correlated with initiation of neuromuscular synaptogenesis

Brian Wolf, Mark A. Seeger, Akira Chiba

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

We show that the Commissureless (COMM) transmembrane protein is required at neuromuscular synaptogenesis. All muscles in the Drosophila embryo express COMM during the period of motoneuron-muscle interaction. It is endocytosed into muscles before synaptogenesis. In comm loss-of-function mutants, motoneuron growth cones fail to initiate synaptogenesis at target muscles. This stall phenotype is rescued by supplying wild-type COMM to the muscles. Cytoplasmically truncated COMM protein fails to internalize. Expressing this mutant protein in muscles phenocopies the synaptogenesis defects of comm mutants. Thus, synaptogenesis initiation is positively correlated with endocytosis of COMM in postsynaptic muscle cells. We propose that COMM is an essential part of the dynamic cell surface remodeling needed by postsynaptic cells in coordinating synaptogenesis initiation.

Original languageEnglish
Pages (from-to)3853-3863
Number of pages11
JournalDevelopment
Volume125
Issue number19
StatePublished - Nov 7 1998
Externally publishedYes

Fingerprint

Endocytosis
Muscles
Motor Neurons
Growth Cones
Mutant Proteins
Muscle Cells
Drosophila
Proteins
Embryonic Structures
Phenotype

Keywords

  • Adaptin
  • Axon
  • Commissureless
  • Drosophila
  • Endocytosis
  • Growth cone
  • Internalization
  • Neuromuscular
  • Synaptogenesis

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Commissureless endocytosis is correlated with initiation of neuromuscular synaptogenesis. / Wolf, Brian; Seeger, Mark A.; Chiba, Akira.

In: Development, Vol. 125, No. 19, 07.11.1998, p. 3853-3863.

Research output: Contribution to journalArticle

Wolf, Brian ; Seeger, Mark A. ; Chiba, Akira. / Commissureless endocytosis is correlated with initiation of neuromuscular synaptogenesis. In: Development. 1998 ; Vol. 125, No. 19. pp. 3853-3863.
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