Combined functions of two RRMs in Dead-end1 mimic helicase activity to promote nanos1 translation in the germline

Tristan Aguero, Zhigang Jin, Dawn Owens, Arun Malhotra, Karen Newman, Jing Yang, Mary Lou King

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Dead-end1 (Dnd1) expression is restricted to the vertebrate germline where it is believed to activate translation of messenger RNAs (mRNAs) required to protect and promote that unique lineage. Nanos1 is one such germline mRNA whose translation is blocked by a secondary mRNA structure within the open reading frame (ORF). Dnd1 contains a canonical RNA recognition motif (RRM1) in its N-terminus but also contains a less conserved RRM2. Here we provide a mechanistic picture of the nanos1 mRNA–Dnd1 interaction in the Xenopus germline. We show that RRM1, but not RRM2, is required for binding nanos1. Similar to the zebrafish homolog, Xenopus Dnd1 possesses ATPase activity. Surprisingly, this activity appears to be within the RRM2, different from the C-terminal region where it is found in zebrafish. More importantly, we show that RRM2 is required for nanos1 translation and germline survival. Further, Dnd1 functions as a homodimer and binds nanos1 mRNA just downstream of the secondary structure required for nanos1 repression. We propose a model in which the RRM1 is required to bind nanos1 mRNA while the RRM2 is required to promote translation through the action of ATPase. Dnd1 appears to use RRMs to mimic the function of helicases.

Original languageEnglish (US)
Pages (from-to)896-908
Number of pages13
JournalMolecular Reproduction and Development
Volume85
Issue number12
DOIs
StatePublished - Dec 2018

Keywords

  • Dead-end1
  • Xenopus
  • germline development
  • nanos1
  • translational regulation

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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