Combined fluorescence and ultrastructural mapping of living cells

Elli Kohen, Joseph G. Hirschberg, Cahide Kohen, Jeffrey Prince, Simon Suckewer, Rene Santus, Patrice Morliere, Louis Dubertret

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A topographic analysis of fluorescence distribution has been carried out pixel-by-pixel by one-dimensional and two-dimensional microspectrofluorometry and three-dimensional confocal fluorescence microscopy. Fluorescence emission spectra of NAD(P)H and benzo(a)pyrene (or metabolites) were recorded at different excitation wavelengths. Cell bioenergetics are monitored in normal and malignant cells as well as cells with genetic defects by coenzyme responses to microinjections of substrates and modifiers from key metabolic pathways in the presence and absence of inhibitors and drugs active on mitochondrial structure and function. Cooperative interactions between organelles involved in detoxification mechanisms are observed in cells treated with fluorescent cytotoxic agents. Such interactions can be directly mapped by the fluorescence of cytotoxic agents, their reaction products or vital probes such as NBD ceramide for the Golgi apparatus. To identify the organelles involved, parallel electron microscopic studies are carried out in cells first treated with the cytotoxic agent and then incubated with an electron opaque material. A recently developed combined X-ray laser microscope (COXRALM) holds the promise of carrying out combined phase-fluorescence-and X-ray microscopic observations of fluorescence and ultrastructural correlations in live cell probing. As further versatility is gained in such methods it may become possible to obtain a very detailed structure and function mapping of living cells within the context of cytomatrix analysis, metabolic compartmentation and organelle interactions.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsJoseph F. Lakowicz
Place of PublicationBellingham, WA, United States
PublisherPubl by Int Soc for Optical Engineering
Pages736-752
Number of pages17
Volume1204 pt 2
ISBN (Print)0819402451
StatePublished - Dec 1 1990
EventTime-Resolved Laser Spectroscopy in Biochemistry II - Los Angeles, CA, USA
Duration: Jan 15 1990Jan 17 1990

Other

OtherTime-Resolved Laser Spectroscopy in Biochemistry II
CityLos Angeles, CA, USA
Period1/15/901/17/90

Fingerprint

Fluorescence
Cells
fluorescence
organelles
cells
Ceramides
Pixels
X ray lasers
Coenzymes
pixels
Detoxification
Electrons
Confocal microscopy
coenzymes
Fluorescence microscopy
Pyrene
Metabolites
Reaction products
metabolites
interactions

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Kohen, E., Hirschberg, J. G., Kohen, C., Prince, J., Suckewer, S., Santus, R., ... Dubertret, L. (1990). Combined fluorescence and ultrastructural mapping of living cells. In J. F. Lakowicz (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1204 pt 2, pp. 736-752). Bellingham, WA, United States: Publ by Int Soc for Optical Engineering.

Combined fluorescence and ultrastructural mapping of living cells. / Kohen, Elli; Hirschberg, Joseph G.; Kohen, Cahide; Prince, Jeffrey; Suckewer, Simon; Santus, Rene; Morliere, Patrice; Dubertret, Louis.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / Joseph F. Lakowicz. Vol. 1204 pt 2 Bellingham, WA, United States : Publ by Int Soc for Optical Engineering, 1990. p. 736-752.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Kohen, E, Hirschberg, JG, Kohen, C, Prince, J, Suckewer, S, Santus, R, Morliere, P & Dubertret, L 1990, Combined fluorescence and ultrastructural mapping of living cells. in JF Lakowicz (ed.), Proceedings of SPIE - The International Society for Optical Engineering. vol. 1204 pt 2, Publ by Int Soc for Optical Engineering, Bellingham, WA, United States, pp. 736-752, Time-Resolved Laser Spectroscopy in Biochemistry II, Los Angeles, CA, USA, 1/15/90.
Kohen E, Hirschberg JG, Kohen C, Prince J, Suckewer S, Santus R et al. Combined fluorescence and ultrastructural mapping of living cells. In Lakowicz JF, editor, Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1204 pt 2. Bellingham, WA, United States: Publ by Int Soc for Optical Engineering. 1990. p. 736-752
Kohen, Elli ; Hirschberg, Joseph G. ; Kohen, Cahide ; Prince, Jeffrey ; Suckewer, Simon ; Santus, Rene ; Morliere, Patrice ; Dubertret, Louis. / Combined fluorescence and ultrastructural mapping of living cells. Proceedings of SPIE - The International Society for Optical Engineering. editor / Joseph F. Lakowicz. Vol. 1204 pt 2 Bellingham, WA, United States : Publ by Int Soc for Optical Engineering, 1990. pp. 736-752
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