Colonization with Escherichia coli EC 25 protects neonatal rats from necrotizing enterocolitis

Debi M. Thomas, Brandon Bell, Stephanie Papillon, Patrick Delaplain, Joanna Lim, Jamie Golden, Jordan Bowling, Jin Wang, Larry Wang, Anatoly V. Grishin, Henri Ford

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Necrotizing enterocolitis (NEC) is a significant cause of morbidity and mortality in premature infants; yet its pathogenesis remains poorly understood. To evaluate the role of intestinal bacteria in protection against NEC, we assessed the ability of naturally occurring intestinal colonizer E. coli EC25 to influence composition of intestinal microbiota and NEC pathology in the neonatal rat model. Experimental NEC was induced in neonatal rats by formula feeding/hypoxia, and graded histologically. Bacterial populations were characterized by plating on blood agar, scoring colony classes, and identifying each class by sequencing 16S rDNA. Binding of bacteria to, and induction of apoptosis in IEC-6 enterocytes were examined by plating on blood agar and fluorescent staining for fragmented DNA. E. coli EC 25, which was originally isolated from healthy rats, efficiently colonized the intestine and protected from NEC following introduction to newborn rats with formula at 106 or 108 cfu. Protection did not depend significantly on EC25 inoculum size or load in the intestine, but positively correlated with the fraction of EC25 in the microbiome. Introduction of EC25 did not prevent colonization with other bacteria and did not significantly alter bacterial diversity. EC25 neither induced cultured enterocyte apoptosis, nor protected from apoptosis induced by an enteropathogenic strain of Cronobacter muytjensii. Our results show that E. coli EC25 is a commensal strain that efficiently colonizes the neonatal intestine and protects from NEC.

Original languageEnglish (US)
Article numbere0188211
JournalPLoS One
Volume12
Issue number11
DOIs
StatePublished - Nov 1 2017
Externally publishedYes

Fingerprint

enterocolitis
Necrotizing Enterocolitis
Escherichia coli
Rats
neonates
Bacteria
Apoptosis
Plating
Agar
Intestines
intestines
Blood
apoptosis
Enterocytes
enterocytes
intestinal microorganisms
Cronobacter
Pathology
Ribosomal DNA
agar

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Colonization with Escherichia coli EC 25 protects neonatal rats from necrotizing enterocolitis. / Thomas, Debi M.; Bell, Brandon; Papillon, Stephanie; Delaplain, Patrick; Lim, Joanna; Golden, Jamie; Bowling, Jordan; Wang, Jin; Wang, Larry; Grishin, Anatoly V.; Ford, Henri.

In: PLoS One, Vol. 12, No. 11, e0188211, 01.11.2017.

Research output: Contribution to journalArticle

Thomas, DM, Bell, B, Papillon, S, Delaplain, P, Lim, J, Golden, J, Bowling, J, Wang, J, Wang, L, Grishin, AV & Ford, H 2017, 'Colonization with Escherichia coli EC 25 protects neonatal rats from necrotizing enterocolitis', PLoS One, vol. 12, no. 11, e0188211. https://doi.org/10.1371/journal.pone.0188211
Thomas DM, Bell B, Papillon S, Delaplain P, Lim J, Golden J et al. Colonization with Escherichia coli EC 25 protects neonatal rats from necrotizing enterocolitis. PLoS One. 2017 Nov 1;12(11). e0188211. https://doi.org/10.1371/journal.pone.0188211
Thomas, Debi M. ; Bell, Brandon ; Papillon, Stephanie ; Delaplain, Patrick ; Lim, Joanna ; Golden, Jamie ; Bowling, Jordan ; Wang, Jin ; Wang, Larry ; Grishin, Anatoly V. ; Ford, Henri. / Colonization with Escherichia coli EC 25 protects neonatal rats from necrotizing enterocolitis. In: PLoS One. 2017 ; Vol. 12, No. 11.
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