Collagenolytic activity and collagen matrix breakdown of the articular cartilage in the pond‐nuki dog model of osteoarthritis

Jean‐Pierre ‐P Pelletier, Johanne Martel‐Pelletier, Roy D. Altman, Latifa Ghandur‐Mnaymneh, David S. Howell, J. Frederick Woessner

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Abstract

Recent reports on the Pond‐Nuki model of osteo‐arthritis in the dog have provided evidence for partial disruption of the collagen network. The possibility of collagenase involvement in these localized changes was studied. Animals were killed 2, 4, 8, and 12 weeks after surgery. The left knee served as a sham‐operated control. Cartilages from femoral condyles were processed for light and electron microscopy and assayed for collagenolytic activity by a direct tissue assay, based on the measurement of digestion of endogenous cartilage collagen. Animals killed at 2 and 4 weeks showed fibrillation and mild erosion of femoral condyles, which usually progressed to ulceration by 8 and 12 weeks. Electron microscopy demonstrated fiber disruption of the midzone perilacunar collagen as early as 2 weeks after the operation. Total collagenolytic activity, measured after activation by aminophenylmercuric acetate, was significantly higher in diseased cartilage than in controls of 2, 4, and 8 weeks; the peak value was at 4 weeks. Collagenase was shown, by its specific action on type I collagen, to be present at 2 and 4 weeks; however, other metallo‐proteases may also contribute to the digestion. The correlation between increased collagenolytic activity and the early osteoarthritic changes in cartilage suggests a role of this enzyme activity in the disease process.

Original languageEnglish (US)
Pages (from-to)866-874
Number of pages9
JournalArthritis & Rheumatism
Volume26
Issue number7
DOIs
StatePublished - Jul 1983

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Rheumatology
  • Immunology
  • Pharmacology (medical)

Cite this

Pelletier, JP. P., Martel‐Pelletier, J., Altman, R. D., Ghandur‐Mnaymneh, L., Howell, D. S., & Frederick Woessner, J. (1983). Collagenolytic activity and collagen matrix breakdown of the articular cartilage in the pond‐nuki dog model of osteoarthritis. Arthritis & Rheumatism, 26(7), 866-874. https://doi.org/10.1002/art.1780260708