Collagenase isoforms for pancreas digestion

Federico Bertuzzi, Silvia Cainarca, Simona Marzorati, Angela Bachi, Barbara Antonioli, Rita Nano, Roberto Verzaro, Camillo Ricordi

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The available information concerning the characteristics and composition of collagenase batches, which are effective in the digestion of human pancreas for islet transplants, is scarce and incomplete. A large interand intrabatched variability in activity and efficiency of blend enzymes available for isolation has been observed. The aim of this study was to characterize enzyme blend components. Liberase batches were characterized by SDS-PAGE analyses, microelectrophoresis, and then by MALDI-TOF MS analysis. Three main bands were detected by SDS-PAGE analysis and submitted to MALDI-TOF MS analysis. Two bands were found to correspond to class I (isoform β and another of 106 kDa) and one to class II (isoform δ) collagenase. These results represent an important step towards a complete characterization of enzymes, with the final aim of identifying key components for a standardized product.

Original languageEnglish (US)
Pages (from-to)203-206
Number of pages4
JournalCell Transplantation
Volume18
Issue number2
DOIs
StatePublished - Jun 16 2009

Keywords

  • Collagenase
  • Islet isolation
  • Proteases
  • Purification

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering

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    Bertuzzi, F., Cainarca, S., Marzorati, S., Bachi, A., Antonioli, B., Nano, R., Verzaro, R., & Ricordi, C. (2009). Collagenase isoforms for pancreas digestion. Cell Transplantation, 18(2), 203-206. https://doi.org/10.3727/096368909788341270