Collagen XII is a regulator of corneal stroma structure and function

Mei Sun, Nabeel Zafrullah, Floriane Devaux, Chinda Hemmavanh, Sheila Adams, Noel M. Ziebarth, Manuel Koch, David E. Birk, Edgar M. Espana

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


PURPOSE. The aim of this study was to determine the roles of collagen XII in the regulation of stromal hierarchical organization, keratocyte organization, and corneal mechanics. METHODS. The temporal and spatial expression of collagen XII at postnatal days 4, 10, 30, 90, and 150 were evaluated in wild-type (WT) mice. The role of collagen XII in hierarchical organization was analyzed by measuring fibril diameter and density, as well as stromal lamellar structure, within ultrastructural micrographs obtained from WT and collagen XII-deficient mice (Col12a1–/–). Keratocyte morphology and networks were assessed using actin staining with phalloidin and in vivo confocal microscopy. The effects of collagen XII on corneal biomechanics were evaluated with atomic force microscopy. RESULTS. Collagen XII was localized homogeneously in the stroma from postnatal day 4 to day 150, and protein accumulation was shown to increase during this period using semiquantitative immunoblots. Higher fibril density (P < 0.001) and disruption of lamellar organization were found in the collagen XII null mice stroma when compared to WT mice. Keratocyte networks and organization were altered in the absence of collagen XII, as demonstrated using fluorescent microscopy after phalloidin staining and in vivo confocal microscopy. Corneal stiffness was increased in the absence of collagen XII. Young’s modulus was 16.2 ± 5.6 kPa in WT and 32.8 ± 6.4 kPa in Col12a1–/– corneas. The difference between these two groups was significant (P < 0.001, t-test). CONCLUSIONS. Collagen XII plays a major role in establishing and maintaining stromal structure and function. In the absence of collagen XII, the corneal stroma showed significant abnormalities, including decreased interfibrillar space, disrupted lamellar organization, abnormal keratocyte organization, and increased corneal stiffness.

Original languageEnglish (US)
Article number61
JournalInvestigative Ophthalmology and Visual Science
Issue number5
StatePublished - May 2020


  • Collagen XII
  • Collagen fibril
  • Development
  • Keratocyte
  • Stroma

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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