Collagen receptors mediate early events in the attachment of epithelial (MDCK) cells

Pedro J.I. Salas, Dora E. Vega-Salas, Enrique Rodriguez-Boulan

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Madin-Darby canine kidney (MDCK) cells kept in suspension culture for 12-15 hr displayed high-affinity binding sites for125I-lathyritic (soluble) collagen (120,000/cell, KD=30 nm) and preferred collagens types I and IV over laminin or fibronectin as substrates during the first hour of attachment. On the other hand, after 4 hr, attachment to all four substrates was equally efficient. Upon challenge with a collagen substrate, the high-affinity sites were rapidly recruited on it (T1/2=6 min). Their occupancy by soluble collagen triggered the exocytosis of a second large population of low-affinity collagen binding sites that included laminin and seems to be involved in a second cell-attachment mechanism. These results are compatible with a twostep model of MDCK cell attachment to the substrate: first, via high-affinity collagen binding sites, and second, via laminin of cellular origin.

Original languageEnglish (US)
Pages (from-to)223-236
Number of pages14
JournalThe Journal of Membrane Biology
Volume98
Issue number3
DOIs
StatePublished - Oct 1 1987
Externally publishedYes

Keywords

  • cell-substrate interaction
  • collagen receptor
  • epithelia
  • epithelial polarity
  • laminin
  • MDCK cells

ASJC Scopus subject areas

  • Physiology
  • Cell Biology
  • Biophysics

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