TY - JOUR
T1 - Coding sequence, genomic organization, and conserved chromosomal localization of the mouse gene Scn11a encoding the sodium channel NaN
AU - Dib-Hajj, Sulayman D.
AU - Tyrrell, Lynda
AU - Escayg, Andrew
AU - Wood, Patrick M.
AU - Meisler, Miriam H.
AU - Waxman, Stephen G.
N1 - Funding Information:
The authors thank Dr. Joel A. Black and William Hormuzadiar for providing mouse trigeminal ganglia. Adult human DRG was kindly provided by Les Olson and the University of Miami Organ Procurement Team. This work was supported in part by grants from the National Multiple Sclerosis Society, the Medical Research Service, Department of Veterans’ Affairs, the Paralyzed Veterans of America, and Eastern Paralyzed Veterans’ Association (S.G.W.), USPHS Grants NS34509 and GM24872 (M.M.), and NINDS Grant NS09923-28 (P.W.).
PY - 1999/8/1
Y1 - 1999/8/1
N2 - Previous studies have shown that sodium channel α-subunit NaN is preferentially expressed in small-diameter sensory neurons of dorsal root ganglia and trigeminal ganglia. These neurons include high-threshold nociceptors that are involved in transduction of pain associated with tissue and nerve injury. In this study, we show that mouse NaN is a 1765-amino-acid peptide that is predicted to produce a current that is resistant to tetrodotoxin (TTX-R). Mouse and rat NaN are 80 and 89% identical at the nucleotide and amino acid levels, respectively. The Scn11a gene encoding this cDNA is organized into 24 exons. Unlike some α-subunits, Scn11a does not have an alternative exon 5 in domain I. Introns of the U2 and U12 spliceosome types are present at conserved positions relative to other members of this family. Scn11a is located on mouse chromosome 9, close to the two other TTX-R sodium channel genes, Scn5a and Scn10a. The human gene, SCN11A, was mapped to the conserved linkage group on chromosome 3p21-p24, close to human SCN5A and SCN10A. The colocalization of the three sodium channel genes supports a common lineage of the TTX-R sodium channels.
AB - Previous studies have shown that sodium channel α-subunit NaN is preferentially expressed in small-diameter sensory neurons of dorsal root ganglia and trigeminal ganglia. These neurons include high-threshold nociceptors that are involved in transduction of pain associated with tissue and nerve injury. In this study, we show that mouse NaN is a 1765-amino-acid peptide that is predicted to produce a current that is resistant to tetrodotoxin (TTX-R). Mouse and rat NaN are 80 and 89% identical at the nucleotide and amino acid levels, respectively. The Scn11a gene encoding this cDNA is organized into 24 exons. Unlike some α-subunits, Scn11a does not have an alternative exon 5 in domain I. Introns of the U2 and U12 spliceosome types are present at conserved positions relative to other members of this family. Scn11a is located on mouse chromosome 9, close to the two other TTX-R sodium channel genes, Scn5a and Scn10a. The human gene, SCN11A, was mapped to the conserved linkage group on chromosome 3p21-p24, close to human SCN5A and SCN10A. The colocalization of the three sodium channel genes supports a common lineage of the TTX-R sodium channels.
UR - http://www.scopus.com/inward/record.url?scp=0033180098&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033180098&partnerID=8YFLogxK
U2 - 10.1006/geno.1999.5890
DO - 10.1006/geno.1999.5890
M3 - Article
C2 - 10444332
AN - SCOPUS:0033180098
VL - 59
SP - 309
EP - 318
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 3
ER -