Cocaine alters Homer1 natural antisense transcript in the nucleus accumbens

Gregory Sartor, Samuel K. Powell, Dmitry Velmeshev, David Y. Lin, Marco Magistri, Hannah J. Wiedner, Andrea M. Malvezzi, Nadja S. Andrade, Mohammad A Faghihi, Claes R Wahlestedt

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Natural antisense transcripts (NATs) are an abundant class of long noncoding RNAs that have recently been shown to be key regulators of chromatin dynamics and gene expression in nervous system development and neurological disorders. However, it is currently unclear if NAT-based mechanisms also play a role in drug-induced neuroadaptations. Aberrant regulation of gene expression is one critical factor underlying the long-lasting behavioral abnormalities that characterize substance use disorder, and it is possible that some drug-induced transcriptional responses are mediated, in part, by perturbations in NAT activity. To test this hypothesis, we used an automated algorithm that mines the NCBI AceView transcriptomics database to identify NAT overlapping genes linked to addiction. We found that 22% of the genes examined contain NATs and that expression of Homer1 natural antisense transcript (Homer1-AS) was altered in the nucleus accumbens (NAc) of mice 2 h and 10 days following repeated cocaine administration. In in vitro studies, depletion of Homer1-AS lead to an increase in the corresponding sense gene expression, indicating a potential regulatory mechanisms of Homer1 expression by its corresponding antisense transcript. Future in vivo studies are needed to definitely determine a role for Homer1-AS in cocaine-induced behavioral and molecular adaptations.

Original languageEnglish (US)
Pages (from-to)183-189
Number of pages7
JournalMolecular and Cellular Neuroscience
Volume85
DOIs
StatePublished - Dec 1 2017

Fingerprint

Nucleus Accumbens
Nervous System Diseases
Cocaine
Overlapping Genes
Long Noncoding RNA
Gene Expression
Gene Expression Regulation
Pharmaceutical Preparations
Chromatin
Substance-Related Disorders
Databases
Genes
In Vitro Techniques

Keywords

  • Cocaine
  • Epigenetics
  • Long noncoding RNA
  • Natural antisense transcripts
  • Nucleus accumbens

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

Cocaine alters Homer1 natural antisense transcript in the nucleus accumbens. / Sartor, Gregory; Powell, Samuel K.; Velmeshev, Dmitry; Lin, David Y.; Magistri, Marco; Wiedner, Hannah J.; Malvezzi, Andrea M.; Andrade, Nadja S.; Faghihi, Mohammad A; Wahlestedt, Claes R.

In: Molecular and Cellular Neuroscience, Vol. 85, 01.12.2017, p. 183-189.

Research output: Contribution to journalArticle

Sartor, G, Powell, SK, Velmeshev, D, Lin, DY, Magistri, M, Wiedner, HJ, Malvezzi, AM, Andrade, NS, Faghihi, MA & Wahlestedt, CR 2017, 'Cocaine alters Homer1 natural antisense transcript in the nucleus accumbens', Molecular and Cellular Neuroscience, vol. 85, pp. 183-189. https://doi.org/10.1016/j.mcn.2017.10.003
Sartor, Gregory ; Powell, Samuel K. ; Velmeshev, Dmitry ; Lin, David Y. ; Magistri, Marco ; Wiedner, Hannah J. ; Malvezzi, Andrea M. ; Andrade, Nadja S. ; Faghihi, Mohammad A ; Wahlestedt, Claes R. / Cocaine alters Homer1 natural antisense transcript in the nucleus accumbens. In: Molecular and Cellular Neuroscience. 2017 ; Vol. 85. pp. 183-189.
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