Co-regulation of constitutive nitric oxide synthases and NADPH oxidase by the small GTPase Rac

Balakrishnan Selvakumar, Douglas T. Hess, Pascal J. Goldschmidt-Clermont, Jonathan S. Stamler

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Nitric oxide (NO), generated by NO synthases (NOSs), has multifarious roles in signal transduction. Reactive oxygen species (ROS), generated by ubiquitous NADPH oxidases (NOXs), also participate in cellular signaling. However, the coordination of signals conveyed by NO and ROS is poorly understood. We show that the small GTPase Rac, a component of some NOXs, also interacts with and regulates the constitutively-expressed NOSs. Cellular NO and O2- production increase or decrease together following activation or inhibition of Rac, and Rac inhibition reveals transduction mechanisms that depend upon NO (vasodilation), ROS (actin polymerization) or both (cytoskeletal organization). Thus, signaling by NO and ROS may be coordinated through a common control element.

Original languageEnglish (US)
Pages (from-to)2195-2202
Number of pages8
JournalFEBS letters
Volume582
Issue number15
DOIs
StatePublished - Jun 25 2008

Keywords

  • NADPH oxidase
  • Nitric oxide
  • Nitric oxide synthase
  • Rac
  • Small GTPase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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