Abstract
Nitric oxide (NO), generated by NO synthases (NOSs), has multifarious roles in signal transduction. Reactive oxygen species (ROS), generated by ubiquitous NADPH oxidases (NOXs), also participate in cellular signaling. However, the coordination of signals conveyed by NO and ROS is poorly understood. We show that the small GTPase Rac, a component of some NOXs, also interacts with and regulates the constitutively-expressed NOSs. Cellular NO and O2- production increase or decrease together following activation or inhibition of Rac, and Rac inhibition reveals transduction mechanisms that depend upon NO (vasodilation), ROS (actin polymerization) or both (cytoskeletal organization). Thus, signaling by NO and ROS may be coordinated through a common control element.
| Original language | English |
|---|---|
| Pages (from-to) | 2195-2202 |
| Number of pages | 8 |
| Journal | FEBS Letters |
| Volume | 582 |
| Issue number | 15 |
| DOIs | |
| State | Published - Jun 25 2008 |
| Externally published | Yes |
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Keywords
- NADPH oxidase
- Nitric oxide
- Nitric oxide synthase
- Rac
- Small GTPase
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Co-regulation of constitutive nitric oxide synthases and NADPH oxidase by the small GTPase Rac. / Selvakumar, Balakrishnan; Hess, Douglas T.; Goldschmidt-Clermont, Pascal; Stamler, Jonathan S.
In: FEBS Letters, Vol. 582, No. 15, 25.06.2008, p. 2195-2202.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Co-regulation of constitutive nitric oxide synthases and NADPH oxidase by the small GTPase Rac
AU - Selvakumar, Balakrishnan
AU - Hess, Douglas T.
AU - Goldschmidt-Clermont, Pascal
AU - Stamler, Jonathan S.
PY - 2008/6/25
Y1 - 2008/6/25
N2 - Nitric oxide (NO), generated by NO synthases (NOSs), has multifarious roles in signal transduction. Reactive oxygen species (ROS), generated by ubiquitous NADPH oxidases (NOXs), also participate in cellular signaling. However, the coordination of signals conveyed by NO and ROS is poorly understood. We show that the small GTPase Rac, a component of some NOXs, also interacts with and regulates the constitutively-expressed NOSs. Cellular NO and O2- production increase or decrease together following activation or inhibition of Rac, and Rac inhibition reveals transduction mechanisms that depend upon NO (vasodilation), ROS (actin polymerization) or both (cytoskeletal organization). Thus, signaling by NO and ROS may be coordinated through a common control element.
AB - Nitric oxide (NO), generated by NO synthases (NOSs), has multifarious roles in signal transduction. Reactive oxygen species (ROS), generated by ubiquitous NADPH oxidases (NOXs), also participate in cellular signaling. However, the coordination of signals conveyed by NO and ROS is poorly understood. We show that the small GTPase Rac, a component of some NOXs, also interacts with and regulates the constitutively-expressed NOSs. Cellular NO and O2- production increase or decrease together following activation or inhibition of Rac, and Rac inhibition reveals transduction mechanisms that depend upon NO (vasodilation), ROS (actin polymerization) or both (cytoskeletal organization). Thus, signaling by NO and ROS may be coordinated through a common control element.
KW - NADPH oxidase
KW - Nitric oxide
KW - Nitric oxide synthase
KW - Rac
KW - Small GTPase
UR - http://www.scopus.com/inward/record.url?scp=44749092029&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=44749092029&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2008.04.062
DO - 10.1016/j.febslet.2008.04.062
M3 - Article
C2 - 18501711
AN - SCOPUS:44749092029
VL - 582
SP - 2195
EP - 2202
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 15
ER -