Co-regulation of constitutive nitric oxide synthases and NADPH oxidase by the small GTPase Rac

Balakrishnan Selvakumar, Douglas T. Hess, Pascal Goldschmidt-Clermont, Jonathan S. Stamler

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Nitric oxide (NO), generated by NO synthases (NOSs), has multifarious roles in signal transduction. Reactive oxygen species (ROS), generated by ubiquitous NADPH oxidases (NOXs), also participate in cellular signaling. However, the coordination of signals conveyed by NO and ROS is poorly understood. We show that the small GTPase Rac, a component of some NOXs, also interacts with and regulates the constitutively-expressed NOSs. Cellular NO and O2- production increase or decrease together following activation or inhibition of Rac, and Rac inhibition reveals transduction mechanisms that depend upon NO (vasodilation), ROS (actin polymerization) or both (cytoskeletal organization). Thus, signaling by NO and ROS may be coordinated through a common control element.

Original languageEnglish
Pages (from-to)2195-2202
Number of pages8
JournalFEBS Letters
Volume582
Issue number15
DOIs
StatePublished - Jun 25 2008
Externally publishedYes

Fingerprint

Monomeric GTP-Binding Proteins
NADPH Oxidase
Nitric Oxide Synthase
Nitric Oxide
Reactive Oxygen Species
Cell signaling
Signal transduction
Vasodilation
Polymerization
Actins
Signal Transduction
Chemical activation

Keywords

  • NADPH oxidase
  • Nitric oxide
  • Nitric oxide synthase
  • Rac
  • Small GTPase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Selvakumar, B., Hess, D. T., Goldschmidt-Clermont, P., & Stamler, J. S. (2008). Co-regulation of constitutive nitric oxide synthases and NADPH oxidase by the small GTPase Rac. FEBS Letters, 582(15), 2195-2202. https://doi.org/10.1016/j.febslet.2008.04.062

Co-regulation of constitutive nitric oxide synthases and NADPH oxidase by the small GTPase Rac. / Selvakumar, Balakrishnan; Hess, Douglas T.; Goldschmidt-Clermont, Pascal; Stamler, Jonathan S.

In: FEBS Letters, Vol. 582, No. 15, 25.06.2008, p. 2195-2202.

Research output: Contribution to journalArticle

Selvakumar, B, Hess, DT, Goldschmidt-Clermont, P & Stamler, JS 2008, 'Co-regulation of constitutive nitric oxide synthases and NADPH oxidase by the small GTPase Rac', FEBS Letters, vol. 582, no. 15, pp. 2195-2202. https://doi.org/10.1016/j.febslet.2008.04.062
Selvakumar, Balakrishnan ; Hess, Douglas T. ; Goldschmidt-Clermont, Pascal ; Stamler, Jonathan S. / Co-regulation of constitutive nitric oxide synthases and NADPH oxidase by the small GTPase Rac. In: FEBS Letters. 2008 ; Vol. 582, No. 15. pp. 2195-2202.
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