Co-registration of radiographic and pathologic infarct territory in a non-human primate model of stroke

Ricardo J Komotar, J. Mocco, William J. Mack, Evan R. Ransom, Brad E. Zacharia, Ryan G. King, Andrew F. Ducruet, Hilary G. Cohen, Victoria Arango, E. Sander Connolly

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objectives: Infarct volume correlation using magnetic resonance imaging (MRI) and pathology specimens enables exact tissue localization of cerebral injury following experimental stroke. We describe a protocol that enables co-registration of radiographic signal change and histologic ischemia in a non-human primate model of stroke. Methods: One male baboon underwent left middle cerebral artery territory occlusion/ reperfusion. MRI [5 mm axial T2 weighted (T2W) slices] was carried out 9 days post-ischemia after which the animal was killed. Immediately post-mortem, the whole brain was perfused and fixed in paraformaldehyde and sliced into 5 mm axial sections that corresponded to those demonstrated on MRI. Slices (40 μm) were obtained from each section and were then stained using Luxol hematoxylin and eosin. Results: The relative area of hyperintensity demonstrated on T2W MRI approximates, in size and location, the region of infarct on gross pathology. This was confirmed microscopically. Discussion: With the use of advanced imaging modalities, this co-registration technique affords the capacity to differentiate ischemic core, penumbra, and uninjured cortex following experimental stroke. Such a precise delineation enables immunohistochemical analysis of a wide variety of substrates in each of the aforementioned regions.

Original languageEnglish
Pages (from-to)634-637
Number of pages4
JournalNeurological Research
Volume27
Issue number6
DOIs
StatePublished - Sep 1 2005
Externally publishedYes

Fingerprint

Primates
Stroke
Magnetic Resonance Imaging
Ischemia
Pathology
Papio
Middle Cerebral Artery Infarction
Hematoxylin
Eosine Yellowish-(YS)
Reperfusion
Wounds and Injuries
Brain

Keywords

  • Histology
  • Magnetic resonance imaging
  • Primate model
  • Stroke

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Komotar, R. J., Mocco, J., Mack, W. J., Ransom, E. R., Zacharia, B. E., King, R. G., ... Connolly, E. S. (2005). Co-registration of radiographic and pathologic infarct territory in a non-human primate model of stroke. Neurological Research, 27(6), 634-637. https://doi.org/10.1179/016164105X17305

Co-registration of radiographic and pathologic infarct territory in a non-human primate model of stroke. / Komotar, Ricardo J; Mocco, J.; Mack, William J.; Ransom, Evan R.; Zacharia, Brad E.; King, Ryan G.; Ducruet, Andrew F.; Cohen, Hilary G.; Arango, Victoria; Connolly, E. Sander.

In: Neurological Research, Vol. 27, No. 6, 01.09.2005, p. 634-637.

Research output: Contribution to journalArticle

Komotar, RJ, Mocco, J, Mack, WJ, Ransom, ER, Zacharia, BE, King, RG, Ducruet, AF, Cohen, HG, Arango, V & Connolly, ES 2005, 'Co-registration of radiographic and pathologic infarct territory in a non-human primate model of stroke', Neurological Research, vol. 27, no. 6, pp. 634-637. https://doi.org/10.1179/016164105X17305
Komotar, Ricardo J ; Mocco, J. ; Mack, William J. ; Ransom, Evan R. ; Zacharia, Brad E. ; King, Ryan G. ; Ducruet, Andrew F. ; Cohen, Hilary G. ; Arango, Victoria ; Connolly, E. Sander. / Co-registration of radiographic and pathologic infarct territory in a non-human primate model of stroke. In: Neurological Research. 2005 ; Vol. 27, No. 6. pp. 634-637.
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