Co-registration of radiographic and pathologic infarct territory in a non-human primate model of stroke

Ricardo J. Komotar, J. Mocco, William J. Mack, Evan R. Ransom, Brad E. Zacharia, Ryan G. King, Andrew F. Ducruet, Hilary G. Cohen, Victoria Arango, E. Sander Connolly

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Objectives: Infarct volume correlation using magnetic resonance imaging (MRI) and pathology specimens enables exact tissue localization of cerebral injury following experimental stroke. We describe a protocol that enables co-registration of radiographic signal change and histologic ischemia in a non-human primate model of stroke. Methods: One male baboon underwent left middle cerebral artery territory occlusion/ reperfusion. MRI [5 mm axial T2 weighted (T2W) slices] was carried out 9 days post-ischemia after which the animal was killed. Immediately post-mortem, the whole brain was perfused and fixed in paraformaldehyde and sliced into 5 mm axial sections that corresponded to those demonstrated on MRI. Slices (40 μm) were obtained from each section and were then stained using Luxol hematoxylin and eosin. Results: The relative area of hyperintensity demonstrated on T2W MRI approximates, in size and location, the region of infarct on gross pathology. This was confirmed microscopically. Discussion: With the use of advanced imaging modalities, this co-registration technique affords the capacity to differentiate ischemic core, penumbra, and uninjured cortex following experimental stroke. Such a precise delineation enables immunohistochemical analysis of a wide variety of substrates in each of the aforementioned regions.

Original languageEnglish (US)
Pages (from-to)634-637
Number of pages4
JournalNeurological Research
Volume27
Issue number6
DOIs
StatePublished - Sep 1 2005
Externally publishedYes

Keywords

  • Histology
  • Magnetic resonance imaging
  • Primate model
  • Stroke

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

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    Komotar, R. J., Mocco, J., Mack, W. J., Ransom, E. R., Zacharia, B. E., King, R. G., Ducruet, A. F., Cohen, H. G., Arango, V., & Connolly, E. S. (2005). Co-registration of radiographic and pathologic infarct territory in a non-human primate model of stroke. Neurological Research, 27(6), 634-637. https://doi.org/10.1179/016164105X17305