TY - JOUR
T1 - Co-ordinate regulation of lactate metabolism genes in yeast
T2 - The role of the lactate permease gene JEN1
AU - Lodi, T.
AU - Fontanesi, F.
AU - Guiard, B.
N1 - Funding Information:
Acknowledgements This research was supported by a grant from the Ministero UniversitaÁ e Ricerca Scientifica e Tecnologica Cofin 2000 to University of Parma and by the French Association pour la Recherche sur le Cancer (ARC). We have grateful to A. Harington for valuable suggestions. We thank D. Menay, who provided the oligonucleotides.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2002/1
Y1 - 2002/1
N2 - In the yeast Saccharomyces cerevisiae, the first step in lactate metabolism is its transport across the plasma membrane, a proton symport process mediated by the product of the gene JEN1. Under aerobic conditions, the expression of JEN1 is regulated by the carbon source: the gene is repressed by glucose and induced by non-fermentable substrates. JEN1 expression is also controlled by oxygen availability, but is unaffected by the absence of haem biosynthesis. JEN1 is negatively regulated by the repressors Mig1p and Mig2p, and requires Cat8p for full derepression. In this report we demonstrate that, in addition to these regulators, the Hap2/3/4/5 complex interacts specifically with a CAAT-box element in the JEN1 promoter, and acts to derepress JEN1 expression. We also provide evidence for transcriptional stimulation of JEN1 by the protein kinase Snf1p. Data are presented which provide a better understanding of the molecular mechanisms implicated in the co-regulation of genes involved in the metabolism of lactate.
AB - In the yeast Saccharomyces cerevisiae, the first step in lactate metabolism is its transport across the plasma membrane, a proton symport process mediated by the product of the gene JEN1. Under aerobic conditions, the expression of JEN1 is regulated by the carbon source: the gene is repressed by glucose and induced by non-fermentable substrates. JEN1 expression is also controlled by oxygen availability, but is unaffected by the absence of haem biosynthesis. JEN1 is negatively regulated by the repressors Mig1p and Mig2p, and requires Cat8p for full derepression. In this report we demonstrate that, in addition to these regulators, the Hap2/3/4/5 complex interacts specifically with a CAAT-box element in the JEN1 promoter, and acts to derepress JEN1 expression. We also provide evidence for transcriptional stimulation of JEN1 by the protein kinase Snf1p. Data are presented which provide a better understanding of the molecular mechanisms implicated in the co-regulation of genes involved in the metabolism of lactate.
KW - Carbon source regulation
KW - JEN1
KW - Lactate metabolism
KW - Oxygen regulation
KW - Yeast
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U2 - 10.1007/s00438-001-0604-y
DO - 10.1007/s00438-001-0604-y
M3 - Article
C2 - 11810259
AN - SCOPUS:0035671476
VL - 266
SP - 838
EP - 847
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
SN - 1617-4615
IS - 5
ER -