Abstract
Background: Polyvalent vaccination represents a recent attempt to improve the effectiveness of lung cancer immunotherapy. This study aimed to investigate whether a gene expression pattern of tumor-associated antigens (TAA) would exist indicating that their use will be most appropriate for the polyvalent vaccination of Caucasian non-small cell lung carcinoma (NSCLC) patients. We examined the concomitant expression of genes belonging to different TAA families for which expression frequencies either have never been detected in NSCLC or vary widely in the literature. Results: 15/23 (65%) and 8/23 (35%) tumor samples were found expressing 6-11 and 2-5 out of the 12 examined TAAs, respectively, at levels >1% of the testis reference sample. The most prevalent TAA patterns observed were those of survivin standard (survivin-std)/survivin-2B expressed by 22/23 (95.5%) tumor samples and of survivin-std/survivin-2B/hTERT expressed by 19/23 (82.5%) tumor samples. The expression levels of the survivin-std gene strongly positively correlated to those of the survivin-2B (p = 0.001) and the hTERT genes (p = 0.031). The number of concomitantly expressed genes was found to be positively correlated to the age of the patients (p = 0.001) and the tumor size (p = 0.048). Methods: Tumor material from 23 patients with NSCLC (12 adenocarcinomas, 8 squamous cell carcinomas, 3 bronchiolo - carcinomas) was examined. mRNA transcripts were detected for 5 genes of the survivin family, 5 MAGE-A genes as well as the genes of human telomerase reverse transcriptase (hTERT) and p53, by the use of quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) or semi-quantitative RT-PCR. Conclusion: This study provides evidence that, in Caucasian patients with NSCLC, highly prevalent expression patterns of TAA genes, predominantly of overexpressed TAAs, do exist. This result implies that the combined use of these TAA could help in designing more effective NSCLC immunotherapeutic protocols.
| Original language | English |
|---|---|
| Pages (from-to) | 345-352 |
| Number of pages | 8 |
| Journal | Cancer Biology and Therapy |
| Volume | 7 |
| Issue number | 3 |
| State | Published - Mar 1 2008 |
| Externally published | Yes |
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Keywords
- Human telomerase reverse transcriptase
- Immunotherapy
- Lung adenocarcinoma
- Melanoma-associated antigen
- p53
- Quantitative real-time PCR
- Squamous cell carcinoma
- Survivin
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Co-expression patterns of tumor-associated antigen genes by non-small cell lung carcinomas : Implications for immunotherapy. / Karanikas, Vaios; Tsochas, Stefanos; Boukas, Konstantinos; Kerenidi, Theodora; Nakou, Marianna; Dahabreh, Jubrail; Poularakis, Theodoros; Gourgoulianis, Konstantinos I.; Germenis, Anastasios E.
In: Cancer Biology and Therapy, Vol. 7, No. 3, 01.03.2008, p. 345-352.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Co-expression patterns of tumor-associated antigen genes by non-small cell lung carcinomas
T2 - Implications for immunotherapy
AU - Karanikas, Vaios
AU - Tsochas, Stefanos
AU - Boukas, Konstantinos
AU - Kerenidi, Theodora
AU - Nakou, Marianna
AU - Dahabreh, Jubrail
AU - Poularakis, Theodoros
AU - Gourgoulianis, Konstantinos I.
AU - Germenis, Anastasios E.
PY - 2008/3/1
Y1 - 2008/3/1
N2 - Background: Polyvalent vaccination represents a recent attempt to improve the effectiveness of lung cancer immunotherapy. This study aimed to investigate whether a gene expression pattern of tumor-associated antigens (TAA) would exist indicating that their use will be most appropriate for the polyvalent vaccination of Caucasian non-small cell lung carcinoma (NSCLC) patients. We examined the concomitant expression of genes belonging to different TAA families for which expression frequencies either have never been detected in NSCLC or vary widely in the literature. Results: 15/23 (65%) and 8/23 (35%) tumor samples were found expressing 6-11 and 2-5 out of the 12 examined TAAs, respectively, at levels >1% of the testis reference sample. The most prevalent TAA patterns observed were those of survivin standard (survivin-std)/survivin-2B expressed by 22/23 (95.5%) tumor samples and of survivin-std/survivin-2B/hTERT expressed by 19/23 (82.5%) tumor samples. The expression levels of the survivin-std gene strongly positively correlated to those of the survivin-2B (p = 0.001) and the hTERT genes (p = 0.031). The number of concomitantly expressed genes was found to be positively correlated to the age of the patients (p = 0.001) and the tumor size (p = 0.048). Methods: Tumor material from 23 patients with NSCLC (12 adenocarcinomas, 8 squamous cell carcinomas, 3 bronchiolo - carcinomas) was examined. mRNA transcripts were detected for 5 genes of the survivin family, 5 MAGE-A genes as well as the genes of human telomerase reverse transcriptase (hTERT) and p53, by the use of quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) or semi-quantitative RT-PCR. Conclusion: This study provides evidence that, in Caucasian patients with NSCLC, highly prevalent expression patterns of TAA genes, predominantly of overexpressed TAAs, do exist. This result implies that the combined use of these TAA could help in designing more effective NSCLC immunotherapeutic protocols.
AB - Background: Polyvalent vaccination represents a recent attempt to improve the effectiveness of lung cancer immunotherapy. This study aimed to investigate whether a gene expression pattern of tumor-associated antigens (TAA) would exist indicating that their use will be most appropriate for the polyvalent vaccination of Caucasian non-small cell lung carcinoma (NSCLC) patients. We examined the concomitant expression of genes belonging to different TAA families for which expression frequencies either have never been detected in NSCLC or vary widely in the literature. Results: 15/23 (65%) and 8/23 (35%) tumor samples were found expressing 6-11 and 2-5 out of the 12 examined TAAs, respectively, at levels >1% of the testis reference sample. The most prevalent TAA patterns observed were those of survivin standard (survivin-std)/survivin-2B expressed by 22/23 (95.5%) tumor samples and of survivin-std/survivin-2B/hTERT expressed by 19/23 (82.5%) tumor samples. The expression levels of the survivin-std gene strongly positively correlated to those of the survivin-2B (p = 0.001) and the hTERT genes (p = 0.031). The number of concomitantly expressed genes was found to be positively correlated to the age of the patients (p = 0.001) and the tumor size (p = 0.048). Methods: Tumor material from 23 patients with NSCLC (12 adenocarcinomas, 8 squamous cell carcinomas, 3 bronchiolo - carcinomas) was examined. mRNA transcripts were detected for 5 genes of the survivin family, 5 MAGE-A genes as well as the genes of human telomerase reverse transcriptase (hTERT) and p53, by the use of quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) or semi-quantitative RT-PCR. Conclusion: This study provides evidence that, in Caucasian patients with NSCLC, highly prevalent expression patterns of TAA genes, predominantly of overexpressed TAAs, do exist. This result implies that the combined use of these TAA could help in designing more effective NSCLC immunotherapeutic protocols.
KW - Human telomerase reverse transcriptase
KW - Immunotherapy
KW - Lung adenocarcinoma
KW - Melanoma-associated antigen
KW - p53
KW - Quantitative real-time PCR
KW - Squamous cell carcinoma
KW - Survivin
UR - http://www.scopus.com/inward/record.url?scp=45349108941&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=45349108941&partnerID=8YFLogxK
M3 - Article
C2 - 18094614
AN - SCOPUS:45349108941
VL - 7
SP - 345
EP - 352
JO - Cancer Biology and Therapy
JF - Cancer Biology and Therapy
SN - 1538-4047
IS - 3
ER -