Co-electrospun dual scaffolding system with potential for muscle-tendon junction tissue engineering

Mitchell R. Ladd, Sang Jin Lee, Joel D. Stitzel, Anthony Atala, James J. Yoo

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

Tissue engineering has had successes developing single tissue types, but there is a need for methods that will allow development of composite tissues. For instance, muscle-tendon junctions (MTJ) require a seamless interface to allow force transfer from muscle to tendon. One challenge in engineering MTJs is designing a continuous scaffold suitable for both tissue types. We aimed to create a dual scaffold that exhibits regional mechanical property differences that mimic the trends seen in native MTJ. Poly(ε-caprolactone)/collagen and poly(l-lactide)/collagen were co-electrospun onto opposite ends of a mandrel to create a scaffold with 3 regions. Scaffolds were characterized with scanning electron microscopy, tensile testing (uniaxial, cyclic, and video strain), for cytocompatibility using MTS, and seeded with C2C12 myoblasts and NIH3T3 fibroblasts. Native porcine diaphragm MTJs were also analyzed with video strain for comparison. Integrated scaffolds were created with fiber diameters from 452-549 nm. Scaffolds exhibited regional variations in mechanical properties with moduli from 4.490-27.62 MPa and generally withstood cyclic testing, although with hysteresis. Video analysis showed scaffold strain profiles exhibited similar trends to native MTJ. The scaffolds were cytocompatible and accommodated cell attachment and myotube formation. The properties engineered into these scaffolds make them attractive candidates for tissue engineering of MTJs.

Original languageEnglish
Pages (from-to)1549-1559
Number of pages11
JournalBiomaterials
Volume32
Issue number6
DOIs
StatePublished - Feb 1 2011
Externally publishedYes

Fingerprint

Tendons
Bioelectric potentials
Tissue Engineering
Tissue engineering
Scaffolds
Muscle
Muscles
Collagen
Myoblasts
Skeletal Muscle Fibers
Tissue
Diaphragm
Electron Scanning Microscopy
Swine
Fibroblasts
Mechanical properties
Tensile testing
Scaffolds (biology)
Diaphragms
Hysteresis

Keywords

  • Composite tissue engineering
  • Electrospinning
  • Mechanical properties
  • Muscle
  • Strain profile
  • Tendon

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Co-electrospun dual scaffolding system with potential for muscle-tendon junction tissue engineering. / Ladd, Mitchell R.; Lee, Sang Jin; Stitzel, Joel D.; Atala, Anthony; Yoo, James J.

In: Biomaterials, Vol. 32, No. 6, 01.02.2011, p. 1549-1559.

Research output: Contribution to journalArticle

Ladd, Mitchell R. ; Lee, Sang Jin ; Stitzel, Joel D. ; Atala, Anthony ; Yoo, James J. / Co-electrospun dual scaffolding system with potential for muscle-tendon junction tissue engineering. In: Biomaterials. 2011 ; Vol. 32, No. 6. pp. 1549-1559.
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