CNTF promotes the survival of neonatal rat corticospinal neurons in vitro

Ella Magal, Jean Claude Louis, Martin Oudega, Silvio Varon

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Corticospinal neurons were identified in cell cultures of neonatal rat cortex by immunostaining of cholera toxin B subunit (CTB), retrogradely transported from the cervical part of the spinal cord. The CTB-immunoreactive neurons were larger than the neurons in the overall (unstained) neuronal population and represented a small fraction of it (average of 0.3%) after 6 hours in vitro. The number of both total and CTB-labeled neurons declined progressively with time in culture. The neuronal death was, however, markedly faster in the CTB-labeled neuronal population than in the overall neuronal population. Ciliary neurotrophic factor (CNTF) promoted the survival of CTB-positive corticospinal neurons in a dose-dependent manner; with CNTF, the death rate of the CTB-labeled neurons became identical to that of the overall population.

Original languageEnglish (US)
Pages (from-to)779-782
Number of pages4
JournalNeuroreport
Volume4
Issue number6
DOIs
StatePublished - Jun 1993
Externally publishedYes

Keywords

  • Cell culture
  • Cntf
  • Corticospinal neuron
  • Neuronal survival

ASJC Scopus subject areas

  • Neuroscience(all)

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