CNTF promotes the survival of neonatal rat corticospinal neurons in vitro

Ella Magal, Jean Claude Louis, Martin Oudega, Silvio Varon

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Corticospinal neurons were identified in cell cultures of neonatal rat cortex by immunostaining of cholera toxin B subunit (CTB), retrogradely transported from the cervical part of the spinal cord. The CTB-immunoreactive neurons were larger than the neurons in the overall (unstained) neuronal population and represented a small fraction of it (average of 0.3%) after 6 hours in vitro. The number of both total and CTB-labeled neurons declined progressively with time in culture. The neuronal death was, however, markedly faster in the CTB-labeled neuronal population than in the overall neuronal population. Ciliary neurotrophic factor (CNTF) promoted the survival of CTB-positive corticospinal neurons in a dose-dependent manner; with CNTF, the death rate of the CTB-labeled neurons became identical to that of the overall population.

Original languageEnglish (US)
Pages (from-to)779-782
Number of pages4
JournalNeuroReport
Volume4
Issue number6
StatePublished - 1993
Externally publishedYes

Fingerprint

Ciliary Neurotrophic Factor
Cholera Toxin
Neurons
Population
In Vitro Techniques
Cell Culture Techniques
Mortality

Keywords

  • Cell culture
  • Cntf
  • Corticospinal neuron
  • Neuronal survival

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Magal, E., Louis, J. C., Oudega, M., & Varon, S. (1993). CNTF promotes the survival of neonatal rat corticospinal neurons in vitro. NeuroReport, 4(6), 779-782.

CNTF promotes the survival of neonatal rat corticospinal neurons in vitro. / Magal, Ella; Louis, Jean Claude; Oudega, Martin; Varon, Silvio.

In: NeuroReport, Vol. 4, No. 6, 1993, p. 779-782.

Research output: Contribution to journalArticle

Magal, E, Louis, JC, Oudega, M & Varon, S 1993, 'CNTF promotes the survival of neonatal rat corticospinal neurons in vitro', NeuroReport, vol. 4, no. 6, pp. 779-782.
Magal, Ella ; Louis, Jean Claude ; Oudega, Martin ; Varon, Silvio. / CNTF promotes the survival of neonatal rat corticospinal neurons in vitro. In: NeuroReport. 1993 ; Vol. 4, No. 6. pp. 779-782.
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