Abstract
In back skin sections from adolescent C57BL/6 mice, regularly distributed, perifollicular inflammatory cell clusters (PICC) were found located around the distal noncycling portion of about 2% of all hair follicles examined. The PICC and the affected hair follicles were characterized during spontaneously developed or induced hair cycle stages, using antibodies against MHC Class II,F4/80, ER-MP23, NLDC 145, CD4, CD8, γδTCR, IL-1 receptor, and ICAM-1. PICC consisted predominantly of macrophages (MAC), accompanied by a few CD4 cells, whereas γδTCR+ and CD8+ cells were absent. During anagen and catagen, some of the PICC hair follicles showed variable degenerative phenomena reminiscent of scarring alopecia: thickened basement membrane, ectopic MHC II expression, MAC infiltration into the follicle epithelium, and signs of keratinocyte apoptosis. Loss of distal outer root sheath keratinocytes was detected in 10% of PICC hair follicles (0.2% of all hair follicles). Because PICC were located in the vicinity of the bulge region, MAC-dependent damage to follicle stem cells might eventually lead to follicle degeneration. These perifollicular MAC clusters around selected hair follicles may indicate the existence of a physiological program of MAC dependent controlled follicle degeneration by which damaged or malfunctioning follicles are removed by programmed organ deletion (POD).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 361-370 |
| Number of pages | 10 |
| Journal | Journal of Histochemistry and Cytochemistry |
| Volume | 46 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 1 1998 |
| Externally published | Yes |
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Keywords
- Alopecia
- Apoptosis
- Hair cycle
- ICAM 1
- Inflammation
- Lymphocyte
- Macrophage
- MHC Class II
ASJC Scopus subject areas
- Anatomy
- Histology
Cite this
Clusters of perifollicular macrophages in normal murine skin : Physiological degeneration of selected hair follicles by programmed organ deletion. / Eichmüller, Stefan; Van Der Veen, Carina; Moll, Ingrid; Hermes, Barbara; Hofmann, Udo; Müller-Röver, Sven; Paus, Ralf.
In: Journal of Histochemistry and Cytochemistry, Vol. 46, No. 3, 01.01.1998, p. 361-370.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Clusters of perifollicular macrophages in normal murine skin
T2 - Physiological degeneration of selected hair follicles by programmed organ deletion
AU - Eichmüller, Stefan
AU - Van Der Veen, Carina
AU - Moll, Ingrid
AU - Hermes, Barbara
AU - Hofmann, Udo
AU - Müller-Röver, Sven
AU - Paus, Ralf
PY - 1998/1/1
Y1 - 1998/1/1
N2 - In back skin sections from adolescent C57BL/6 mice, regularly distributed, perifollicular inflammatory cell clusters (PICC) were found located around the distal noncycling portion of about 2% of all hair follicles examined. The PICC and the affected hair follicles were characterized during spontaneously developed or induced hair cycle stages, using antibodies against MHC Class II,F4/80, ER-MP23, NLDC 145, CD4, CD8, γδTCR, IL-1 receptor, and ICAM-1. PICC consisted predominantly of macrophages (MAC), accompanied by a few CD4 cells, whereas γδTCR+ and CD8+ cells were absent. During anagen and catagen, some of the PICC hair follicles showed variable degenerative phenomena reminiscent of scarring alopecia: thickened basement membrane, ectopic MHC II expression, MAC infiltration into the follicle epithelium, and signs of keratinocyte apoptosis. Loss of distal outer root sheath keratinocytes was detected in 10% of PICC hair follicles (0.2% of all hair follicles). Because PICC were located in the vicinity of the bulge region, MAC-dependent damage to follicle stem cells might eventually lead to follicle degeneration. These perifollicular MAC clusters around selected hair follicles may indicate the existence of a physiological program of MAC dependent controlled follicle degeneration by which damaged or malfunctioning follicles are removed by programmed organ deletion (POD).
AB - In back skin sections from adolescent C57BL/6 mice, regularly distributed, perifollicular inflammatory cell clusters (PICC) were found located around the distal noncycling portion of about 2% of all hair follicles examined. The PICC and the affected hair follicles were characterized during spontaneously developed or induced hair cycle stages, using antibodies against MHC Class II,F4/80, ER-MP23, NLDC 145, CD4, CD8, γδTCR, IL-1 receptor, and ICAM-1. PICC consisted predominantly of macrophages (MAC), accompanied by a few CD4 cells, whereas γδTCR+ and CD8+ cells were absent. During anagen and catagen, some of the PICC hair follicles showed variable degenerative phenomena reminiscent of scarring alopecia: thickened basement membrane, ectopic MHC II expression, MAC infiltration into the follicle epithelium, and signs of keratinocyte apoptosis. Loss of distal outer root sheath keratinocytes was detected in 10% of PICC hair follicles (0.2% of all hair follicles). Because PICC were located in the vicinity of the bulge region, MAC-dependent damage to follicle stem cells might eventually lead to follicle degeneration. These perifollicular MAC clusters around selected hair follicles may indicate the existence of a physiological program of MAC dependent controlled follicle degeneration by which damaged or malfunctioning follicles are removed by programmed organ deletion (POD).
KW - Alopecia
KW - Apoptosis
KW - Hair cycle
KW - ICAM 1
KW - Inflammation
KW - Lymphocyte
KW - Macrophage
KW - MHC Class II
UR - http://www.scopus.com/inward/record.url?scp=0031883889&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031883889&partnerID=8YFLogxK
U2 - 10.1177/002215549804600310
DO - 10.1177/002215549804600310
M3 - Article
C2 - 9487118
AN - SCOPUS:0031883889
VL - 46
SP - 361
EP - 370
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
SN - 0022-1554
IS - 3
ER -