Clusters of perifollicular macrophages in normal murine skin: Physiological degeneration of selected hair follicles by programmed organ deletion

Stefan Eichmüller, Carina Van Der Veen, Ingrid Moll, Barbara Hermes, Udo Hofmann, Sven Müller-Röver, Ralf Paus

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

In back skin sections from adolescent C57BL/6 mice, regularly distributed, perifollicular inflammatory cell clusters (PICC) were found located around the distal noncycling portion of about 2% of all hair follicles examined. The PICC and the affected hair follicles were characterized during spontaneously developed or induced hair cycle stages, using antibodies against MHC Class II,F4/80, ER-MP23, NLDC 145, CD4, CD8, γδTCR, IL-1 receptor, and ICAM-1. PICC consisted predominantly of macrophages (MAC), accompanied by a few CD4 cells, whereas γδTCR+ and CD8+ cells were absent. During anagen and catagen, some of the PICC hair follicles showed variable degenerative phenomena reminiscent of scarring alopecia: thickened basement membrane, ectopic MHC II expression, MAC infiltration into the follicle epithelium, and signs of keratinocyte apoptosis. Loss of distal outer root sheath keratinocytes was detected in 10% of PICC hair follicles (0.2% of all hair follicles). Because PICC were located in the vicinity of the bulge region, MAC-dependent damage to follicle stem cells might eventually lead to follicle degeneration. These perifollicular MAC clusters around selected hair follicles may indicate the existence of a physiological program of MAC dependent controlled follicle degeneration by which damaged or malfunctioning follicles are removed by programmed organ deletion (POD).

Original languageEnglish (US)
Pages (from-to)361-370
Number of pages10
JournalJournal of Histochemistry and Cytochemistry
Volume46
Issue number3
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

Fingerprint

Hair Follicle
Macrophages
Skin
Keratinocytes
Interleukin-1 Receptors
Alopecia
Intercellular Adhesion Molecule-1
Inbred C57BL Mouse
Basement Membrane
Hair
Cicatrix
Stem Cells
Epithelium
Apoptosis
Antibodies

Keywords

  • Alopecia
  • Apoptosis
  • Hair cycle
  • ICAM 1
  • Inflammation
  • Lymphocyte
  • Macrophage
  • MHC Class II

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Clusters of perifollicular macrophages in normal murine skin : Physiological degeneration of selected hair follicles by programmed organ deletion. / Eichmüller, Stefan; Van Der Veen, Carina; Moll, Ingrid; Hermes, Barbara; Hofmann, Udo; Müller-Röver, Sven; Paus, Ralf.

In: Journal of Histochemistry and Cytochemistry, Vol. 46, No. 3, 01.01.1998, p. 361-370.

Research output: Contribution to journalArticle

Eichmüller, Stefan ; Van Der Veen, Carina ; Moll, Ingrid ; Hermes, Barbara ; Hofmann, Udo ; Müller-Röver, Sven ; Paus, Ralf. / Clusters of perifollicular macrophages in normal murine skin : Physiological degeneration of selected hair follicles by programmed organ deletion. In: Journal of Histochemistry and Cytochemistry. 1998 ; Vol. 46, No. 3. pp. 361-370.
@article{0fbe568dd3ab45f9a05cbcf6fff862de,
title = "Clusters of perifollicular macrophages in normal murine skin: Physiological degeneration of selected hair follicles by programmed organ deletion",
abstract = "In back skin sections from adolescent C57BL/6 mice, regularly distributed, perifollicular inflammatory cell clusters (PICC) were found located around the distal noncycling portion of about 2{\%} of all hair follicles examined. The PICC and the affected hair follicles were characterized during spontaneously developed or induced hair cycle stages, using antibodies against MHC Class II,F4/80, ER-MP23, NLDC 145, CD4, CD8, γδTCR, IL-1 receptor, and ICAM-1. PICC consisted predominantly of macrophages (MAC), accompanied by a few CD4 cells, whereas γδTCR+ and CD8+ cells were absent. During anagen and catagen, some of the PICC hair follicles showed variable degenerative phenomena reminiscent of scarring alopecia: thickened basement membrane, ectopic MHC II expression, MAC infiltration into the follicle epithelium, and signs of keratinocyte apoptosis. Loss of distal outer root sheath keratinocytes was detected in 10{\%} of PICC hair follicles (0.2{\%} of all hair follicles). Because PICC were located in the vicinity of the bulge region, MAC-dependent damage to follicle stem cells might eventually lead to follicle degeneration. These perifollicular MAC clusters around selected hair follicles may indicate the existence of a physiological program of MAC dependent controlled follicle degeneration by which damaged or malfunctioning follicles are removed by programmed organ deletion (POD).",
keywords = "Alopecia, Apoptosis, Hair cycle, ICAM 1, Inflammation, Lymphocyte, Macrophage, MHC Class II",
author = "Stefan Eichm{\"u}ller and {Van Der Veen}, Carina and Ingrid Moll and Barbara Hermes and Udo Hofmann and Sven M{\"u}ller-R{\"o}ver and Ralf Paus",
year = "1998",
month = "1",
day = "1",
doi = "10.1177/002215549804600310",
language = "English (US)",
volume = "46",
pages = "361--370",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "3",

}

TY - JOUR

T1 - Clusters of perifollicular macrophages in normal murine skin

T2 - Physiological degeneration of selected hair follicles by programmed organ deletion

AU - Eichmüller, Stefan

AU - Van Der Veen, Carina

AU - Moll, Ingrid

AU - Hermes, Barbara

AU - Hofmann, Udo

AU - Müller-Röver, Sven

AU - Paus, Ralf

PY - 1998/1/1

Y1 - 1998/1/1

N2 - In back skin sections from adolescent C57BL/6 mice, regularly distributed, perifollicular inflammatory cell clusters (PICC) were found located around the distal noncycling portion of about 2% of all hair follicles examined. The PICC and the affected hair follicles were characterized during spontaneously developed or induced hair cycle stages, using antibodies against MHC Class II,F4/80, ER-MP23, NLDC 145, CD4, CD8, γδTCR, IL-1 receptor, and ICAM-1. PICC consisted predominantly of macrophages (MAC), accompanied by a few CD4 cells, whereas γδTCR+ and CD8+ cells were absent. During anagen and catagen, some of the PICC hair follicles showed variable degenerative phenomena reminiscent of scarring alopecia: thickened basement membrane, ectopic MHC II expression, MAC infiltration into the follicle epithelium, and signs of keratinocyte apoptosis. Loss of distal outer root sheath keratinocytes was detected in 10% of PICC hair follicles (0.2% of all hair follicles). Because PICC were located in the vicinity of the bulge region, MAC-dependent damage to follicle stem cells might eventually lead to follicle degeneration. These perifollicular MAC clusters around selected hair follicles may indicate the existence of a physiological program of MAC dependent controlled follicle degeneration by which damaged or malfunctioning follicles are removed by programmed organ deletion (POD).

AB - In back skin sections from adolescent C57BL/6 mice, regularly distributed, perifollicular inflammatory cell clusters (PICC) were found located around the distal noncycling portion of about 2% of all hair follicles examined. The PICC and the affected hair follicles were characterized during spontaneously developed or induced hair cycle stages, using antibodies against MHC Class II,F4/80, ER-MP23, NLDC 145, CD4, CD8, γδTCR, IL-1 receptor, and ICAM-1. PICC consisted predominantly of macrophages (MAC), accompanied by a few CD4 cells, whereas γδTCR+ and CD8+ cells were absent. During anagen and catagen, some of the PICC hair follicles showed variable degenerative phenomena reminiscent of scarring alopecia: thickened basement membrane, ectopic MHC II expression, MAC infiltration into the follicle epithelium, and signs of keratinocyte apoptosis. Loss of distal outer root sheath keratinocytes was detected in 10% of PICC hair follicles (0.2% of all hair follicles). Because PICC were located in the vicinity of the bulge region, MAC-dependent damage to follicle stem cells might eventually lead to follicle degeneration. These perifollicular MAC clusters around selected hair follicles may indicate the existence of a physiological program of MAC dependent controlled follicle degeneration by which damaged or malfunctioning follicles are removed by programmed organ deletion (POD).

KW - Alopecia

KW - Apoptosis

KW - Hair cycle

KW - ICAM 1

KW - Inflammation

KW - Lymphocyte

KW - Macrophage

KW - MHC Class II

UR - http://www.scopus.com/inward/record.url?scp=0031883889&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031883889&partnerID=8YFLogxK

U2 - 10.1177/002215549804600310

DO - 10.1177/002215549804600310

M3 - Article

C2 - 9487118

AN - SCOPUS:0031883889

VL - 46

SP - 361

EP - 370

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 3

ER -