Cloning of polymorphisms (COP): enrichment of polymorphic sequences from complex genomes

Jingfeng Li, Fuli Wang, Veronika Zabarovska, Claes Wahlestedt, Eugene R. Zabarovsky

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Here we describe a new procedure (cloning of polymorphisms, COP) for enrichment of single nucleotide polymorphisms (SNPs) that represent restriction fragment length polymorphisms (RFLPs). COP would be applicable to the isolation of SNPs from particular regions of the genome, e.g. CpG islands, chromosomal bands, YACs or PAC contigs. A combination of digestion with restriction enzymes, treatment with uracil-DNA glycosylase and mung bean nuclease, PCR amplification and purification with streptavidin magnetic beads was used to isolate polymorphic sequences from the genomes of two human samples. After only two cycles of enrichment, 80% of the isolated clones were found to contain RFLPs. A simple method for the PCR detection of these polymorphisms was also developed.

Original languageEnglish (US)
Number of pages1
JournalNucleic acids research
Volume28
Issue number2
StatePublished - Jan 15 2000
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Cloning of polymorphisms (COP): enrichment of polymorphic sequences from complex genomes'. Together they form a unique fingerprint.

  • Cite this