CD30 is a member of the TNF/NGF receptor family with an unknown function. We have used the yeast two hybrid system to study proteins involved in CD30 signalling. With baits derived from portions of the murine CD30 intracellular domain, several partial cDNAs from a murine T cell lymphoma library were cloned which encode proteins that specifically interact with the CD30 bait. These cDNAs have no homology to known genes although some have homologous human Expressed Sequence Tags. The cDNA for clone 45-5-3 was used as a Northern hybridization probe against a panel of mouse tissues and cell lines. Clone 45-5-3 encodes an mRNA that is 1.3 kB long and is expressed ubiquitously in all tissues and cell lines tested. We are currently using 5' and 3' PCR RACE to clone the entire coding sequences for this and seven other CD30 interacting protein genes. Hisefusion proteins of truncated CD30 intracellular domains have been generated and are being used in in vitro interaction studies with in vitro transcription/translation protein products generated from our cDNA sequences to verify the yeast two hybrid interactions. These cloned CD30 interacting proteins will help elucidate the function and regulation of CD30 signalling.
|Original language||English (US)|
|State||Published - Dec 1 1996|
ASJC Scopus subject areas
- Molecular Biology