TY - JOUR
T1 - Cloning of Human Brain and Mouse Brain cDNAs Coding for S1, the Second Member of the Mammalian Elongation Factor-1 alpha Gene Family
T2 - Analysis of a Possible Evolutionary Pathway
AU - Lee, S.
AU - Ann, D. K.
AU - Wang, E.
PY - 1994/9/30
Y1 - 1994/9/30
N2 - We previously reported the cloning of a rat S1 cDNA whose deduced amino acid sequence shares high similarity (92%) with that of mammalian elongation factor-1 alpha (EF-1α), a protein involved in the binding of aminoacyl-tRNA to the ribosome during peptide synthesis. We report here the isolation of a full-length cDNA from a mouse brain library and a partial-length cDNA from a human hippocampus library which share extensive sequence similarity to rat S1 cDNA. We show that, as with mammalian EF-1αs, the predicted primary amino acid sequences of rat, mouse, and human S1 are almost identical, except for one conservative substitution. These results indicate that mouse and man contain a second member of the EF-1α gene family, the S1 gene. They also suggest that our result obtained in rat may be extrapolated to mouse and man.
AB - We previously reported the cloning of a rat S1 cDNA whose deduced amino acid sequence shares high similarity (92%) with that of mammalian elongation factor-1 alpha (EF-1α), a protein involved in the binding of aminoacyl-tRNA to the ribosome during peptide synthesis. We report here the isolation of a full-length cDNA from a mouse brain library and a partial-length cDNA from a human hippocampus library which share extensive sequence similarity to rat S1 cDNA. We show that, as with mammalian EF-1αs, the predicted primary amino acid sequences of rat, mouse, and human S1 are almost identical, except for one conservative substitution. These results indicate that mouse and man contain a second member of the EF-1α gene family, the S1 gene. They also suggest that our result obtained in rat may be extrapolated to mouse and man.
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U2 - 10.1006/bbrc.1994.2336
DO - 10.1006/bbrc.1994.2336
M3 - Article
C2 - 7945283
AN - SCOPUS:0028061407
VL - 203
SP - 1371
EP - 1377
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -