Cloning, expression, purification and characterization of human endostatin gene

Fang Yang, Yuan Li He, Xiaoyu Jiang, Yun Liu, Dong Xian Peng, Li Li Zong

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To procure biologically active human endostatin. METHODS: Human endostatin gene was acquired by means of reverse transcriptase (RT)-PCR and cloned into PGEM-T vector with subsequent sequence identification. The gene fragment was inserted into the prokaryotic expression vector pBV220 and transformed into E.coli DH5alpha strain. Endostatin expression in the E.coli was identified and the inclusion body isolated, purified and its activity analyzed. RESULTS: The obtained gene fragment 552 bp in length was identified as the functional section of human endostatin gene by sequence analysis, and SDS-PAGE analysis showed that the expressed product was the target protein with biological activity. CONCLUSION: Human endostatin gene was expressed in E.coli and the protein obtained can inhibit the proliferation of ECV 304 cells.

Original languageEnglish (US)
Pages (from-to)416-418
Number of pages3
JournalDi 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
Volume25
Issue number4
StatePublished - Apr 2005
Externally publishedYes

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Endostatins
Organism Cloning
Genes
Escherichia coli
Escherichia coli Proteins
Inclusion Bodies
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis
Polyacrylamide Gel Electrophoresis
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cloning, expression, purification and characterization of human endostatin gene. / Yang, Fang; He, Yuan Li; Jiang, Xiaoyu; Liu, Yun; Peng, Dong Xian; Zong, Li Li.

In: Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA, Vol. 25, No. 4, 04.2005, p. 416-418.

Research output: Contribution to journalArticle

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