Cloning, expression, and characterization of the gsdA gene encoding thermophilic glucose-6-phosphate dehydrogenase from Aquifex aeolicus

Ramesh B. Iyer, Jianquan Wang, Leonidas G. Bachas

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The gsdA gene of the extreme thermophilic bacterium Aquifex aeolicus, encoding glucose-6-phosphate dehydrogenase (G6PDH), was cloned into a high-expression vector and overexpressed as a fusion protein in Escherichia coli. Here we report the characterization of this recombinant thermostable G6PDH. G6PDH was purified to homogeneity by heat precipitation followed by immobilized metal affinity chromatography on a nickel-chelate column. The data obtained indicate that the enzyme is a homodimer with a subunit molecular weight of 55 kDa. G6PDH followed Michaelis-Menten kinetics with a KM of 63 μM for glucose-6-phosphate at 70°C with NADP as the cofactor. The enzyme exhibited dual coenzyme specificity, although it showed a preference in terms of kcat/KM of 20.4-fold for NADP over NAD at 40°C and 5.7-fold at 70°C. The enzyme showed optimum catalytic activity at 90°C. Modeling of the dimer interface suggested the presence of cysteine residues that may form disulfide bonds between the two subunits, thereby preserving the oligomeric integrity of the enzyme. Interestingly, addition of dithiothreitol or mercaptoethanol did not affect the activity of the enzyme. With a half-life of 24 h at 90°C and 12 h at 100°C, this is the most thermostable G6PDH described.

Original languageEnglish (US)
Pages (from-to)283-289
Number of pages7
JournalExtremophiles
Volume6
Issue number4
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

Keywords

  • Aquifex aeolicus
  • Cloning
  • Enzyme
  • Glucose-6-phosphate dehydrogenase
  • Thermophilic
  • Thermostability

ASJC Scopus subject areas

  • Microbiology
  • Molecular Medicine

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