Cloning and evaluation of the role of rat GALR-2, a novel subtype of galanin receptor, in the control of pain perception

Sultan Ahmad, Dajan O'Donnell, Kem Payza, Julie Ducharme, Daniel Ménard, William Brown, Ralf Schmidt, Claes R Wahlestedt, S. H. Shen, Philippe Walker

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

We have identified a novel subtype of galanin receptor (GALR-2) in rat dorsal root ganglia and spinal cord. The open reading frame of GALR-2 is 1116 nucleotides long, encoding a protein of 372 amino acids with a theoretical molecular mass of 40.7 kD. Membranes prepared from stable pools of 293 cells expressing GALR-2, but not wild-type 293 cells, demonstrated high affinity galanin binding sites. Rat galanin and galanin-related peptides M40, C7, M15, and galanin1-16 effectively competed for binding; peptide C7 demonstrated a lower affinity for rGALR-2, and all these peptides were agonists at rGALR-2 when assessed on a microphysiometer. Studies on the expression of GALR-2 in various tissues by Northern and in situ hybridization analyses suggest a low abundance but wide distribution of GALR-2 mRNA, including several discrete areas in brain and spinal cord and a high abundance in the dorsal root ganglia.

Original languageEnglish
Pages (from-to)108-119
Number of pages12
JournalAnnals of the New York Academy of Sciences
Volume863
DOIs
StatePublished - Dec 1 1998
Externally publishedYes

Fingerprint

Galanin Receptors
Galanin
Pain Perception
Cloning
Rats
Organism Cloning
Spinal Ganglia
Receptor, Galanin, Type 2
Spinal Cord
Peptides
Molecular mass
Open Reading Frames
In Situ Hybridization
Brain
Nucleotides
Binding Sites
Cells
Tissue
Membranes
Amino Acids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Cloning and evaluation of the role of rat GALR-2, a novel subtype of galanin receptor, in the control of pain perception. / Ahmad, Sultan; O'Donnell, Dajan; Payza, Kem; Ducharme, Julie; Ménard, Daniel; Brown, William; Schmidt, Ralf; Wahlestedt, Claes R; Shen, S. H.; Walker, Philippe.

In: Annals of the New York Academy of Sciences, Vol. 863, 01.12.1998, p. 108-119.

Research output: Contribution to journalArticle

Ahmad, Sultan ; O'Donnell, Dajan ; Payza, Kem ; Ducharme, Julie ; Ménard, Daniel ; Brown, William ; Schmidt, Ralf ; Wahlestedt, Claes R ; Shen, S. H. ; Walker, Philippe. / Cloning and evaluation of the role of rat GALR-2, a novel subtype of galanin receptor, in the control of pain perception. In: Annals of the New York Academy of Sciences. 1998 ; Vol. 863. pp. 108-119.
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AU - Ménard, Daniel

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