Cloning and evaluation of the role of rat GALR-2, a novel subtype of galanin receptor, in the control of pain perception

Sultan Ahmad, Dajan O'Donnell, Kem Payza, Julie Ducharme, Daniel Ménard, William Brown, Ralf Schmidt, Claes Wahlestedt, S. H. Shen, Philippe Walker

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

We have identified a novel subtype of galanin receptor (GALR-2) in rat dorsal root ganglia and spinal cord. The open reading frame of GALR-2 is 1116 nucleotides long, encoding a protein of 372 amino acids with a theoretical molecular mass of 40.7 kD. Membranes prepared from stable pools of 293 cells expressing GALR-2, but not wild-type 293 cells, demonstrated high affinity galanin binding sites. Rat galanin and galanin-related peptides M40, C7, M15, and galanin1-16 effectively competed for binding; peptide C7 demonstrated a lower affinity for rGALR-2, and all these peptides were agonists at rGALR-2 when assessed on a microphysiometer. Studies on the expression of GALR-2 in various tissues by Northern and in situ hybridization analyses suggest a low abundance but wide distribution of GALR-2 mRNA, including several discrete areas in brain and spinal cord and a high abundance in the dorsal root ganglia.

Original languageEnglish (US)
Pages (from-to)108-119
Number of pages12
JournalAnnals of the New York Academy of Sciences
Volume863
DOIs
StatePublished - 1998

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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