Cloning and chromosomal localization of the gene encoding human cyclin D-binding Myb-like protein (hDMP1)

Sara M. Bodner, Clayton W. Naeve, Karen M. Rakestraw, Bart G. Jones, Virginia A. Valentine, Marcus B. Valentine, Frederick W. Luthardt, Cheryl L. Willman, Susana C. Raimondi, James R. Downing, Martine F. Roussel, Charles J. Sherr, A. Thomas Look

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The murine transcription factor murine cyclin D-binding Myb-like protein (mDmp1) arrests the cell cycle in G1 phase, through an activity that can be overridden by direct interaction with the D-type cyclins. Here, we describe the identification, sequence, chromosomal localization, and expression of the human cognate, hDMP1. The hDMP1 cDNA contains a 2280 bp open reading frame that shares a high degree of identity with the mDmpI coding region. The 4.4 kb hDMP1 messenger RNA is ubiquitously expressed in normal human tissues, with highest levels in testis and substructures within the brain. By use of fluorescence in situ hybridization with a human genomic P1 probe, we assigned hDMP1 to chromosome 7, band q21. This chromosomal region is frequently deleted as part of the 7q-minus and monosomy 7 abnormalities of human acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). We analyzed hDMP1 copy number by fluorescence in situ hybridization in leukemic blasts from nine patients with abnormalities of the long arm of chromosome 7, and in each case one allele of the hDMP1 gene was deleted. Functional analysis of the mDmp1 protein has shown that it negatively regulates cell proliferation, which suggests that this gene is a candidate suppressor of malignant transformation. Further study will be needed to determine whether gene- specific mutations implicate hDMP1 as a tumor suppressor in acute leukemias with deletions of the long arm of chromosome 7 or in other types of human malignancy.

Original languageEnglish
Pages (from-to)223-228
Number of pages6
JournalGene
Volume229
Issue number1-2
DOIs
StatePublished - Mar 18 1999

Fingerprint

Organism Cloning
Chromosomes, Human, Pair 7
Cyclin D
Genes
Fluorescence In Situ Hybridization
Myelodysplastic Syndromes
G1 Phase
Cell Cycle Checkpoints
Acute Myeloid Leukemia
Open Reading Frames
Testis
Neoplasms
Leukemia
Proteins
Transcription Factors
Complementary DNA
Alleles
Cell Proliferation
human DMTF1 protein
Messenger RNA

Keywords

  • Cell cycle regulation
  • Myelodysplastic syndrome
  • Transcription factor

ASJC Scopus subject areas

  • Genetics

Cite this

Bodner, S. M., Naeve, C. W., Rakestraw, K. M., Jones, B. G., Valentine, V. A., Valentine, M. B., ... Look, A. T. (1999). Cloning and chromosomal localization of the gene encoding human cyclin D-binding Myb-like protein (hDMP1). Gene, 229(1-2), 223-228. https://doi.org/10.1016/S0378-1119(98)00591-5

Cloning and chromosomal localization of the gene encoding human cyclin D-binding Myb-like protein (hDMP1). / Bodner, Sara M.; Naeve, Clayton W.; Rakestraw, Karen M.; Jones, Bart G.; Valentine, Virginia A.; Valentine, Marcus B.; Luthardt, Frederick W.; Willman, Cheryl L.; Raimondi, Susana C.; Downing, James R.; Roussel, Martine F.; Sherr, Charles J.; Look, A. Thomas.

In: Gene, Vol. 229, No. 1-2, 18.03.1999, p. 223-228.

Research output: Contribution to journalArticle

Bodner, SM, Naeve, CW, Rakestraw, KM, Jones, BG, Valentine, VA, Valentine, MB, Luthardt, FW, Willman, CL, Raimondi, SC, Downing, JR, Roussel, MF, Sherr, CJ & Look, AT 1999, 'Cloning and chromosomal localization of the gene encoding human cyclin D-binding Myb-like protein (hDMP1)', Gene, vol. 229, no. 1-2, pp. 223-228. https://doi.org/10.1016/S0378-1119(98)00591-5
Bodner SM, Naeve CW, Rakestraw KM, Jones BG, Valentine VA, Valentine MB et al. Cloning and chromosomal localization of the gene encoding human cyclin D-binding Myb-like protein (hDMP1). Gene. 1999 Mar 18;229(1-2):223-228. https://doi.org/10.1016/S0378-1119(98)00591-5
Bodner, Sara M. ; Naeve, Clayton W. ; Rakestraw, Karen M. ; Jones, Bart G. ; Valentine, Virginia A. ; Valentine, Marcus B. ; Luthardt, Frederick W. ; Willman, Cheryl L. ; Raimondi, Susana C. ; Downing, James R. ; Roussel, Martine F. ; Sherr, Charles J. ; Look, A. Thomas. / Cloning and chromosomal localization of the gene encoding human cyclin D-binding Myb-like protein (hDMP1). In: Gene. 1999 ; Vol. 229, No. 1-2. pp. 223-228.
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AU - Willman, Cheryl L.

AU - Raimondi, Susana C.

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