Abstract
The product of the human oncogene ELL encodes an RNA polymerase II transcription factor that undergoes frequent translocation in acute myeloid leukemia (AML). In addition to its elongation activity, ELL contains a novel type of RNA polymerase H interaction domain that is capable of repressing polymerase activity in promoter-specific transcription. Remarkably, the ELL translocation that is found in patients with AML results in the deletion of exactly this functional domain. Here we report that the EAP30 subunit of the ELL complex has sequence homology to the Saccharomyces cerevisiae SNF8, whose genetic analysis suggests its involvement in in the derepression of gene expression. Remarkably, EAP30 can interact ELL and derepress ELL's inhibitory activity in vitro. This finding may reveal a key role for EAP30 in the pathogenesis of human leukemia.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 21981-21985 |
| Number of pages | 5 |
| Journal | Journal of Biological Chemistry |
| Volume | 274 |
| Issue number | 31 |
| DOIs | |
| State | Published - Jul 30 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
Cite this
Cloning and characterization of the EAP30 subunit of the ELL complex that confers derepression of transcription by RNA polymerase II. / Schmidt, Amy E.; Miller, Trissa; Schmidt, Susan L.; Shiekhattar, Ramin; Shilatifard, Ali.
In: Journal of Biological Chemistry, Vol. 274, No. 31, 30.07.1999, p. 21981-21985.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning and characterization of the EAP30 subunit of the ELL complex that confers derepression of transcription by RNA polymerase II
AU - Schmidt, Amy E.
AU - Miller, Trissa
AU - Schmidt, Susan L.
AU - Shiekhattar, Ramin
AU - Shilatifard, Ali
PY - 1999/7/30
Y1 - 1999/7/30
N2 - The product of the human oncogene ELL encodes an RNA polymerase II transcription factor that undergoes frequent translocation in acute myeloid leukemia (AML). In addition to its elongation activity, ELL contains a novel type of RNA polymerase H interaction domain that is capable of repressing polymerase activity in promoter-specific transcription. Remarkably, the ELL translocation that is found in patients with AML results in the deletion of exactly this functional domain. Here we report that the EAP30 subunit of the ELL complex has sequence homology to the Saccharomyces cerevisiae SNF8, whose genetic analysis suggests its involvement in in the derepression of gene expression. Remarkably, EAP30 can interact ELL and derepress ELL's inhibitory activity in vitro. This finding may reveal a key role for EAP30 in the pathogenesis of human leukemia.
AB - The product of the human oncogene ELL encodes an RNA polymerase II transcription factor that undergoes frequent translocation in acute myeloid leukemia (AML). In addition to its elongation activity, ELL contains a novel type of RNA polymerase H interaction domain that is capable of repressing polymerase activity in promoter-specific transcription. Remarkably, the ELL translocation that is found in patients with AML results in the deletion of exactly this functional domain. Here we report that the EAP30 subunit of the ELL complex has sequence homology to the Saccharomyces cerevisiae SNF8, whose genetic analysis suggests its involvement in in the derepression of gene expression. Remarkably, EAP30 can interact ELL and derepress ELL's inhibitory activity in vitro. This finding may reveal a key role for EAP30 in the pathogenesis of human leukemia.
UR - http://www.scopus.com/inward/record.url?scp=0033618430&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033618430&partnerID=8YFLogxK
U2 - 10.1074/jbc.274.31.21981
DO - 10.1074/jbc.274.31.21981
M3 - Article
C2 - 10419521
AN - SCOPUS:0033618430
VL - 274
SP - 21981
EP - 21985
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 31
ER -