Cloning and characterization of SLP3: A novel member of the stomatin family expressed by olfactory receptor neurons

Bradley J Goldstein, Heather M. Kulaga, Randall R. Reed

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The detection of odorants with high sensitivity and specificity utilizes specialized transduction proteins that may be assembled into complexes to afford enhanced speed and efficiency in olfactory neurons. We have used a differential cDNA screening technique to identify novel gene products that display restricted expression within the olfactory epithelium. Here we report the characterization of an olfactory neuronal protein, SLP3, which shares extensive homology with the stomatin family of membrane proteins. Other stomatin family members have been implicated in specific interactions with ion channels and G proteincoupled receptors. The pattern of SLP3 mRNA expression during embryonic development and the subcellular localization of the SLP3 protein in mature olfactory neurons observed here is consistent with a specific role for this protein in the assembly, translocation, or function of the odorant transduction complex in olfactory neurons.

Original languageEnglish
Pages (from-to)74-82
Number of pages9
JournalJARO - Journal of the Association for Research in Otolaryngology
Volume4
Issue number1
DOIs
StatePublished - Mar 1 2003
Externally publishedYes

Fingerprint

Olfactory Receptor Neurons
Organism Cloning
Neurons
Proteins
Olfactory Mucosa
Ion Channels
Embryonic Development
Membrane Proteins
Complementary DNA
Sensitivity and Specificity
Messenger RNA
Genes
Odorants

Keywords

  • Neuron
  • Olfaction
  • Stomatin
  • Transduction

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Physiology

Cite this

Cloning and characterization of SLP3 : A novel member of the stomatin family expressed by olfactory receptor neurons. / Goldstein, Bradley J; Kulaga, Heather M.; Reed, Randall R.

In: JARO - Journal of the Association for Research in Otolaryngology, Vol. 4, No. 1, 01.03.2003, p. 74-82.

Research output: Contribution to journalArticle

@article{9bd41866d7e344c0a2d296f4e32f701f,
title = "Cloning and characterization of SLP3: A novel member of the stomatin family expressed by olfactory receptor neurons",
abstract = "The detection of odorants with high sensitivity and specificity utilizes specialized transduction proteins that may be assembled into complexes to afford enhanced speed and efficiency in olfactory neurons. We have used a differential cDNA screening technique to identify novel gene products that display restricted expression within the olfactory epithelium. Here we report the characterization of an olfactory neuronal protein, SLP3, which shares extensive homology with the stomatin family of membrane proteins. Other stomatin family members have been implicated in specific interactions with ion channels and G proteincoupled receptors. The pattern of SLP3 mRNA expression during embryonic development and the subcellular localization of the SLP3 protein in mature olfactory neurons observed here is consistent with a specific role for this protein in the assembly, translocation, or function of the odorant transduction complex in olfactory neurons.",
keywords = "Neuron, Olfaction, Stomatin, Transduction",
author = "Goldstein, {Bradley J} and Kulaga, {Heather M.} and Reed, {Randall R.}",
year = "2003",
month = "3",
day = "1",
doi = "10.1007/s10162-002-2039-5",
language = "English",
volume = "4",
pages = "74--82",
journal = "JARO - Journal of the Association for Research in Otolaryngology",
issn = "1525-3961",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Cloning and characterization of SLP3

T2 - A novel member of the stomatin family expressed by olfactory receptor neurons

AU - Goldstein, Bradley J

AU - Kulaga, Heather M.

AU - Reed, Randall R.

PY - 2003/3/1

Y1 - 2003/3/1

N2 - The detection of odorants with high sensitivity and specificity utilizes specialized transduction proteins that may be assembled into complexes to afford enhanced speed and efficiency in olfactory neurons. We have used a differential cDNA screening technique to identify novel gene products that display restricted expression within the olfactory epithelium. Here we report the characterization of an olfactory neuronal protein, SLP3, which shares extensive homology with the stomatin family of membrane proteins. Other stomatin family members have been implicated in specific interactions with ion channels and G proteincoupled receptors. The pattern of SLP3 mRNA expression during embryonic development and the subcellular localization of the SLP3 protein in mature olfactory neurons observed here is consistent with a specific role for this protein in the assembly, translocation, or function of the odorant transduction complex in olfactory neurons.

AB - The detection of odorants with high sensitivity and specificity utilizes specialized transduction proteins that may be assembled into complexes to afford enhanced speed and efficiency in olfactory neurons. We have used a differential cDNA screening technique to identify novel gene products that display restricted expression within the olfactory epithelium. Here we report the characterization of an olfactory neuronal protein, SLP3, which shares extensive homology with the stomatin family of membrane proteins. Other stomatin family members have been implicated in specific interactions with ion channels and G proteincoupled receptors. The pattern of SLP3 mRNA expression during embryonic development and the subcellular localization of the SLP3 protein in mature olfactory neurons observed here is consistent with a specific role for this protein in the assembly, translocation, or function of the odorant transduction complex in olfactory neurons.

KW - Neuron

KW - Olfaction

KW - Stomatin

KW - Transduction

UR - http://www.scopus.com/inward/record.url?scp=0037347094&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037347094&partnerID=8YFLogxK

U2 - 10.1007/s10162-002-2039-5

DO - 10.1007/s10162-002-2039-5

M3 - Article

C2 - 12239636

AN - SCOPUS:0037347094

VL - 4

SP - 74

EP - 82

JO - JARO - Journal of the Association for Research in Otolaryngology

JF - JARO - Journal of the Association for Research in Otolaryngology

SN - 1525-3961

IS - 1

ER -