TY - JOUR
T1 - Cloning and characterization of Hepp, a novel gene expressed preferentially in hematopoietic progenitors and mature blood cells
AU - Abdullah, James M.
AU - Jing, Xin
AU - Spassov, Danislav S.
AU - Nachtman, Ronald G.
AU - Jurecic, Roland
AU - Jurecic, Roland
N1 - Funding Information:
We thank Dr. Ihor Lemischka (Princeton University) for a generous gift of Sca-1+c-kit+AA4.1+Lin−, AA4.1−, and Rh-123lowSca-1+c-kit+Lin− cell-specific cDNA libraries. This work was partially supported by the Tumor Immunology and Immunotherapy Program of the University of Miami Sylvester Comprehensive Cancer Center and the Stanley Glaser Research Foundation.
PY - 2001
Y1 - 2001
N2 - Through differential screening of mouse hematopoietic stem cell (HSC) and progenitor-subtracted cDNA libraries we have identified a progenitor cell-specific transcript that represents a novel gene, named Hepp (hematopoietic progenitor protein). The mouse Hepp gene encodes a protein of 237 amino acids with no detectable known functional domains or motifs. Lack of invertebrate orthologs and a high degree of evolutionary conservation of the peptide sequence in vertebrate species (zebrafish, mouse, human) suggest that the Hepp gene could have conserved although as yet unknown function in vertebrates. Mouse Hepp shows a restricted expression pattern in adult tissues and is transcribed at a very low level in heart, lung, spleen, and thymus and at a higher level in muscle. During embryonic hematopoiesis Hepp is not expressed in mouse fetal liver HSC (Sca-1+ c-kit+ AA4.1+Lin- cells), but is abundantly transcribed in the population of hematopoietic progenitors (AA4.1- cells). Similarly, during adult hematopoiesis Hepp is not transcribed in the highly enriched population of bone marrow HSC (Rh-123lowSca-1+c-kit+Lin- cells), but its expression is upregulated as a greater heterogeneous population of bone marrow HSC (Lin-Sca-1+ cells) differentiates into progenitors (Lin-Sca-1- cells) and more mature lymphoid and myeloid cell types. A restricted pattern of expression in adult tissues and preferential expression in both fetal and adult hematopoietic progenitors and mature blood cells suggest that Hepp could be involved in molecular regulation of HSC and progenitor cell lineage commitment and differentiation.
AB - Through differential screening of mouse hematopoietic stem cell (HSC) and progenitor-subtracted cDNA libraries we have identified a progenitor cell-specific transcript that represents a novel gene, named Hepp (hematopoietic progenitor protein). The mouse Hepp gene encodes a protein of 237 amino acids with no detectable known functional domains or motifs. Lack of invertebrate orthologs and a high degree of evolutionary conservation of the peptide sequence in vertebrate species (zebrafish, mouse, human) suggest that the Hepp gene could have conserved although as yet unknown function in vertebrates. Mouse Hepp shows a restricted expression pattern in adult tissues and is transcribed at a very low level in heart, lung, spleen, and thymus and at a higher level in muscle. During embryonic hematopoiesis Hepp is not expressed in mouse fetal liver HSC (Sca-1+ c-kit+ AA4.1+Lin- cells), but is abundantly transcribed in the population of hematopoietic progenitors (AA4.1- cells). Similarly, during adult hematopoiesis Hepp is not transcribed in the highly enriched population of bone marrow HSC (Rh-123lowSca-1+c-kit+Lin- cells), but its expression is upregulated as a greater heterogeneous population of bone marrow HSC (Lin-Sca-1+ cells) differentiates into progenitors (Lin-Sca-1- cells) and more mature lymphoid and myeloid cell types. A restricted pattern of expression in adult tissues and preferential expression in both fetal and adult hematopoietic progenitors and mature blood cells suggest that Hepp could be involved in molecular regulation of HSC and progenitor cell lineage commitment and differentiation.
KW - Differential gene expression
KW - Hematopoietic stem cells hematopoietic progenitors
KW - Hepp
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U2 - 10.1006/bcmd.2001.0434
DO - 10.1006/bcmd.2001.0434
M3 - Article
C2 - 11482882
AN - SCOPUS:0034879618
VL - 27
SP - 667
EP - 676
JO - Blood Cells, Molecules, and Diseases
JF - Blood Cells, Molecules, and Diseases
SN - 1079-9796
IS - 3
ER -