Cloning and characterization of an acidic cytolysin cDNA from sea anemone Sagartia rosea

Xiaoyu Jiang, Wen Li Yang, Hui Ping Chen, Hong Bin Tu, Wen Yan Wu, Jian Wen Wei, Juan Wang, Wen Hua Liu, An Long Xu

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A full-length cDNA of cytolysin (Src I) was isolated from the tentacle of Sagartia rosea (a representative species in China) by reverse transcription polymerase chain reaction. The cDNA with an open reading frame of 648bp encodes a precursor protein of 216 amino acids, which contains a prepropeptide of 38 amino acids including a signal peptide of 19 amino acids and a propart of 19 amino acids. Lys-Pro at C-terminus of propart is a cleavage site for proline-endopeptidase-like protease. The mature cytolysin has a molecular mass of 19.6kDa and a pI value of 4.8. Src I is an acidic cytolysin found in sea anemone and shares 75% amino acid sequence similarity to equinatoxin II (Eqt II). The predicted secondary structure of the mature cytolysin comprises 15% α-helix, 45% β-sheet, and 40% random coil. The characteristic amphiphilic α-helix of cytolysin is located at the N-terminus of the processed Src I.

Original languageEnglish (US)
Pages (from-to)1563-1569
Number of pages7
JournalToxicon
Volume40
Issue number11
DOIs
StatePublished - Nov 1 2002
Externally publishedYes

Fingerprint

Sea Anemones
Perforin
Cloning
Organism Cloning
Complementary DNA
Amino Acids
prolyl oligopeptidase
lysylproline
Protein Precursors
Polymerase chain reaction
Molecular mass
Transcription
Protein Sorting Signals
Open Reading Frames
Reverse Transcription
Amino Acid Sequence
China
Peptide Hydrolases
Polymerase Chain Reaction

Keywords

  • Acidic cytolysin
  • Cloning
  • Sagartia rosea
  • Sea anemone
  • Src I

ASJC Scopus subject areas

  • Toxicology

Cite this

Jiang, X., Yang, W. L., Chen, H. P., Tu, H. B., Wu, W. Y., Wei, J. W., ... Xu, A. L. (2002). Cloning and characterization of an acidic cytolysin cDNA from sea anemone Sagartia rosea. Toxicon, 40(11), 1563-1569. https://doi.org/10.1016/S0041-0101(02)00173-3

Cloning and characterization of an acidic cytolysin cDNA from sea anemone Sagartia rosea. / Jiang, Xiaoyu; Yang, Wen Li; Chen, Hui Ping; Tu, Hong Bin; Wu, Wen Yan; Wei, Jian Wen; Wang, Juan; Liu, Wen Hua; Xu, An Long.

In: Toxicon, Vol. 40, No. 11, 01.11.2002, p. 1563-1569.

Research output: Contribution to journalArticle

Jiang, X, Yang, WL, Chen, HP, Tu, HB, Wu, WY, Wei, JW, Wang, J, Liu, WH & Xu, AL 2002, 'Cloning and characterization of an acidic cytolysin cDNA from sea anemone Sagartia rosea', Toxicon, vol. 40, no. 11, pp. 1563-1569. https://doi.org/10.1016/S0041-0101(02)00173-3
Jiang, Xiaoyu ; Yang, Wen Li ; Chen, Hui Ping ; Tu, Hong Bin ; Wu, Wen Yan ; Wei, Jian Wen ; Wang, Juan ; Liu, Wen Hua ; Xu, An Long. / Cloning and characterization of an acidic cytolysin cDNA from sea anemone Sagartia rosea. In: Toxicon. 2002 ; Vol. 40, No. 11. pp. 1563-1569.
@article{feeeec16197f4a15975e6e6b7fa4c738,
title = "Cloning and characterization of an acidic cytolysin cDNA from sea anemone Sagartia rosea",
abstract = "A full-length cDNA of cytolysin (Src I) was isolated from the tentacle of Sagartia rosea (a representative species in China) by reverse transcription polymerase chain reaction. The cDNA with an open reading frame of 648bp encodes a precursor protein of 216 amino acids, which contains a prepropeptide of 38 amino acids including a signal peptide of 19 amino acids and a propart of 19 amino acids. Lys-Pro at C-terminus of propart is a cleavage site for proline-endopeptidase-like protease. The mature cytolysin has a molecular mass of 19.6kDa and a pI value of 4.8. Src I is an acidic cytolysin found in sea anemone and shares 75{\%} amino acid sequence similarity to equinatoxin II (Eqt II). The predicted secondary structure of the mature cytolysin comprises 15{\%} α-helix, 45{\%} β-sheet, and 40{\%} random coil. The characteristic amphiphilic α-helix of cytolysin is located at the N-terminus of the processed Src I.",
keywords = "Acidic cytolysin, Cloning, Sagartia rosea, Sea anemone, Src I",
author = "Xiaoyu Jiang and Yang, {Wen Li} and Chen, {Hui Ping} and Tu, {Hong Bin} and Wu, {Wen Yan} and Wei, {Jian Wen} and Juan Wang and Liu, {Wen Hua} and Xu, {An Long}",
year = "2002",
month = "11",
day = "1",
doi = "10.1016/S0041-0101(02)00173-3",
language = "English (US)",
volume = "40",
pages = "1563--1569",
journal = "Toxicon",
issn = "0041-0101",
publisher = "Elsevier Limited",
number = "11",

}

TY - JOUR

T1 - Cloning and characterization of an acidic cytolysin cDNA from sea anemone Sagartia rosea

AU - Jiang, Xiaoyu

AU - Yang, Wen Li

AU - Chen, Hui Ping

AU - Tu, Hong Bin

AU - Wu, Wen Yan

AU - Wei, Jian Wen

AU - Wang, Juan

AU - Liu, Wen Hua

AU - Xu, An Long

PY - 2002/11/1

Y1 - 2002/11/1

N2 - A full-length cDNA of cytolysin (Src I) was isolated from the tentacle of Sagartia rosea (a representative species in China) by reverse transcription polymerase chain reaction. The cDNA with an open reading frame of 648bp encodes a precursor protein of 216 amino acids, which contains a prepropeptide of 38 amino acids including a signal peptide of 19 amino acids and a propart of 19 amino acids. Lys-Pro at C-terminus of propart is a cleavage site for proline-endopeptidase-like protease. The mature cytolysin has a molecular mass of 19.6kDa and a pI value of 4.8. Src I is an acidic cytolysin found in sea anemone and shares 75% amino acid sequence similarity to equinatoxin II (Eqt II). The predicted secondary structure of the mature cytolysin comprises 15% α-helix, 45% β-sheet, and 40% random coil. The characteristic amphiphilic α-helix of cytolysin is located at the N-terminus of the processed Src I.

AB - A full-length cDNA of cytolysin (Src I) was isolated from the tentacle of Sagartia rosea (a representative species in China) by reverse transcription polymerase chain reaction. The cDNA with an open reading frame of 648bp encodes a precursor protein of 216 amino acids, which contains a prepropeptide of 38 amino acids including a signal peptide of 19 amino acids and a propart of 19 amino acids. Lys-Pro at C-terminus of propart is a cleavage site for proline-endopeptidase-like protease. The mature cytolysin has a molecular mass of 19.6kDa and a pI value of 4.8. Src I is an acidic cytolysin found in sea anemone and shares 75% amino acid sequence similarity to equinatoxin II (Eqt II). The predicted secondary structure of the mature cytolysin comprises 15% α-helix, 45% β-sheet, and 40% random coil. The characteristic amphiphilic α-helix of cytolysin is located at the N-terminus of the processed Src I.

KW - Acidic cytolysin

KW - Cloning

KW - Sagartia rosea

KW - Sea anemone

KW - Src I

UR - http://www.scopus.com/inward/record.url?scp=0036844454&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036844454&partnerID=8YFLogxK

U2 - 10.1016/S0041-0101(02)00173-3

DO - 10.1016/S0041-0101(02)00173-3

M3 - Article

C2 - 12419507

AN - SCOPUS:0036844454

VL - 40

SP - 1563

EP - 1569

JO - Toxicon

JF - Toxicon

SN - 0041-0101

IS - 11

ER -