Cloning and analysis of microRNAs encoded by the primate γ-herpesvirus rhesus monkey rhadinovirus

Alexandra Schäfer, Xuezhong Cai, John P. Bilello, Ronald C. Desrosiers, Bryan R. Cullen

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

Several pathogenic human herpesviruses have recently been shown to express virally encoded microRNAs in infected cells. Although the function of these microRNAs is largely unknown, they are hypothesized to play a role in mediating viral replication by downregulating cellular mRNAs encoding antiviral factors. Here, we report the cloning and analysis of microRNAs encoded by Rhesus Monkey Rhadinovirus (RRV), an animal virus model for the pathogenic human γ-herpesvirus Kaposi's Sarcoma-Associated Herpesvirus (KSHV). RRV expresses several microRNAs that are encoded in the same genomic location as the previously reported KSHV microRNAs, yet these microRNAs are unrelated in primary sequence. These data set the stage for the mutational ablation and phenotypic analysis of RRV mutants lacking one or more viral microRNAs.

Original languageEnglish (US)
Pages (from-to)21-27
Number of pages7
JournalVirology
Volume364
Issue number1
DOIs
StatePublished - Jul 20 2007

Keywords

  • Kaposi's Sarcoma-Associated Herpesvirus
  • Rhesus monkey rhadinovirus
  • microRNAs

ASJC Scopus subject areas

  • Virology

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