Clonal, self-renewing and differentiating human and porcine urothelial cells, a novel stem cell population

Hans M. Larsson, Francois Gorostidi, Jeffrey A. Hubbell, Yann Barrandon, Peter Frey

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Although urothelial progenitor-like cells have been described in the human urinary tract, the existence of stem cells remains to be proven. Using a culture system that favors clonogenic epithelial cell growth, we evaluated and characterized clonal human urothelial cells. We isolated human urothelial cells that were clonogenic, capable of self-renewal and could develop into fully differentiated urothelium once re-implanted into the subcapsular space of nude mice. In addition to final urothelial cell differentiation, spontaneous formation of bladder-like microstructures was observed. By examining an epithelial stem cell signature marker, we found p63 to correlate with the self-renewal capacity of the isolated human urothelial clonal populations. Since a clinically relevant, long-term model for functional reconstitution of human cells does not exist, we sought to establish a culture method for porcine urothelial cells in a clinically relevant porcine model. We isolated cells from porcine ureter, urethra and bladder that were clonogenic and capable of self-renewal and differentiation into fully mature urothelium. In conclusion, we could isolate human and porcine cell populations, behaving as urothelial stem cells and showing clonogenicity, self-renewal and, once re-implanted, morphological differentiation.

Original languageEnglish
Article numbere90006
JournalPLoS One
Volume9
Issue number2
DOIs
StatePublished - Feb 26 2014
Externally publishedYes

Fingerprint

Stem cells
stem cells
Swine
Stem Cells
swine
Cells
Population
Urothelium
Cell growth
cells
Cell culture
bladder
Urinary Bladder
Epithelial Cells
epithelial cells
Microstructure
ureter
Urethra
urethra
Ureter

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Clonal, self-renewing and differentiating human and porcine urothelial cells, a novel stem cell population. / Larsson, Hans M.; Gorostidi, Francois; Hubbell, Jeffrey A.; Barrandon, Yann; Frey, Peter.

In: PLoS One, Vol. 9, No. 2, e90006, 26.02.2014.

Research output: Contribution to journalArticle

Larsson, Hans M. ; Gorostidi, Francois ; Hubbell, Jeffrey A. ; Barrandon, Yann ; Frey, Peter. / Clonal, self-renewing and differentiating human and porcine urothelial cells, a novel stem cell population. In: PLoS One. 2014 ; Vol. 9, No. 2.
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