Clonal CD5-positive B lymphocytes in myelodysplastic syndrome with systemic vasculitis and trisomy 8

R. Billström, B. Johansson, B. Strömbeck, W. Ei-Rifai, M. Larramendy, T. Olofsson, F. Mitelman, S. Knuutila

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Bone marrow and peripheral blood from a myelodysplastic syndrome (MDS) patient with trisomy 8 and associated systemic vasculitis was investigated for clonal lymphoid lineage involvement using simultaneous metaphase and interphase fluorescence in situ hybridization (FISH) and immunocytochemistry with antibodies against CD13 (granulocytic), glycophorin A (GPA, erythroid), and the lymphocytic antigens CD3, CD5, CD20, and CD22. Trisomy 8 was detected in 55% of CD13+, 40% of GPA+, 6% of CD5+, and 5% of CD20/22+, but not in CD3+ cells. In a complementary experiment using interphase FISH on bone marrow cells sorted by flow cytometry, 13% of CD5/CD19 double-positive cells (76% purity) were found to be trisomic. The results indicate the existence of a small CD5-positive B-lymphoid clone as part of the MDS process in this patient. Since CD5/19-positive cells have been proposed to be autoantibody producing, this finding might be a clue to the pathogenesis underlying the propensity for MDS patients to develop immune-mediated complications.

Original languageEnglish (US)
Pages (from-to)37-40
Number of pages4
JournalAnnals of Hematology
Volume74
Issue number1
DOIs
StatePublished - 1997
Externally publishedYes

Keywords

  • CD5/CD19
  • Lymphoid cells
  • MDS
  • Trisomy 8
  • Vasculitis

ASJC Scopus subject areas

  • Hematology

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