Clinicoimmunopathologic findings in Atlantic bottlenose dolphins Tursiops truncatus with positive Chlamydiaceae antibody titers

Gregory D. Bossart, Tracy A. Romano, Margie M. Peden-Adams, Adam Schaefer, Stephen McCulloch, Juli D. Goldstein, Charles D. Rice, Patricia A. Fair, Carolyn Cray, John S. Reif

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Sera from free-ranging Atlantic bottlenose dolphins Tursiops truncatus inhabiting the Indian River Lagoon (IRL), Florida, and coastal waters of Charleston (CHS), South Carolina, USA, were tested for antibodies to Chlamydiaceae as part of a multidisciplinary study of individual and population health. A suite of clinicoimmunopathologic variables was evaluated in Chlamydiaceae-seropositive dolphins (n = 43) and seronegative healthy dolphins (n = 83). Fibrinogen, lactate dehydrogenase, amylase, and absolute numbers of neutrophils, lymphocytes, and basophils were significantly higher, and serum bicarbonate, total alpha globulin, and alpha-2 globulin were significantly lower in dolphins with positive Chlamydiaceae titers compared with seronegative healthy dolphins. Several differences in markers of innate and adaptive immunity were also found. Concanavalin A-induced T lymphocyte proliferation, lipopolysaccharide-induced B lymphocyte proliferation, and granulocytic phagocytosis were significantly lower, and absolute numbers of mature CD 21 B lymphocytes, natural killer cell activity and lysozyme concentration were significantly higher in dolphins with positive Chlamydiaceae antibody titers compared to seronegative healthy dolphins. Additionally, dolphins with positive Chlamydiaceae antibody titers had significant increases in ELISA antibody titers to Erysipelothrix rhusiopathiae. These data suggest that Chlamydiaceae infection may produce subclinical clinicoimmunopathologic perturbations that impact health. Any potential subclinical health impacts are important for the IRL and CHS dolphin populations, as past studies have indicated that both dolphin populations are affected by other complex infectious and neoplastic diseases, often associated with immunologic perturbations and anthropogenic contaminants.

Original languageEnglish
Pages (from-to)71-81
Number of pages11
JournalDiseases of Aquatic Organisms
Volume108
Issue number1
DOIs
StatePublished - Feb 4 2014

Fingerprint

Chlamydiaceae
Tursiops truncatus
dolphin
dolphins
antibody
antibodies
lymphocyte proliferation
blood serum
health impact
B-lymphocytes
serum
lagoon
alpha-globulins
Erysipelothrix rhusiopathiae
perturbation
basophils
rivers
natural killer cells
fibrinogen
concanavalin A

Keywords

  • Bottlenose dolphin
  • Chlamydiaceae antibody
  • Clinical pathology
  • Immunology
  • Seroepidemiology

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology, Evolution, Behavior and Systematics

Cite this

Bossart, G. D., Romano, T. A., Peden-Adams, M. M., Schaefer, A., McCulloch, S., Goldstein, J. D., ... Reif, J. S. (2014). Clinicoimmunopathologic findings in Atlantic bottlenose dolphins Tursiops truncatus with positive Chlamydiaceae antibody titers. Diseases of Aquatic Organisms, 108(1), 71-81. https://doi.org/10.3354/dao02704

Clinicoimmunopathologic findings in Atlantic bottlenose dolphins Tursiops truncatus with positive Chlamydiaceae antibody titers. / Bossart, Gregory D.; Romano, Tracy A.; Peden-Adams, Margie M.; Schaefer, Adam; McCulloch, Stephen; Goldstein, Juli D.; Rice, Charles D.; Fair, Patricia A.; Cray, Carolyn; Reif, John S.

In: Diseases of Aquatic Organisms, Vol. 108, No. 1, 04.02.2014, p. 71-81.

Research output: Contribution to journalArticle

Bossart, GD, Romano, TA, Peden-Adams, MM, Schaefer, A, McCulloch, S, Goldstein, JD, Rice, CD, Fair, PA, Cray, C & Reif, JS 2014, 'Clinicoimmunopathologic findings in Atlantic bottlenose dolphins Tursiops truncatus with positive Chlamydiaceae antibody titers', Diseases of Aquatic Organisms, vol. 108, no. 1, pp. 71-81. https://doi.org/10.3354/dao02704
Bossart, Gregory D. ; Romano, Tracy A. ; Peden-Adams, Margie M. ; Schaefer, Adam ; McCulloch, Stephen ; Goldstein, Juli D. ; Rice, Charles D. ; Fair, Patricia A. ; Cray, Carolyn ; Reif, John S. / Clinicoimmunopathologic findings in Atlantic bottlenose dolphins Tursiops truncatus with positive Chlamydiaceae antibody titers. In: Diseases of Aquatic Organisms. 2014 ; Vol. 108, No. 1. pp. 71-81.
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abstract = "Sera from free-ranging Atlantic bottlenose dolphins Tursiops truncatus inhabiting the Indian River Lagoon (IRL), Florida, and coastal waters of Charleston (CHS), South Carolina, USA, were tested for antibodies to Chlamydiaceae as part of a multidisciplinary study of individual and population health. A suite of clinicoimmunopathologic variables was evaluated in Chlamydiaceae-seropositive dolphins (n = 43) and seronegative healthy dolphins (n = 83). Fibrinogen, lactate dehydrogenase, amylase, and absolute numbers of neutrophils, lymphocytes, and basophils were significantly higher, and serum bicarbonate, total alpha globulin, and alpha-2 globulin were significantly lower in dolphins with positive Chlamydiaceae titers compared with seronegative healthy dolphins. Several differences in markers of innate and adaptive immunity were also found. Concanavalin A-induced T lymphocyte proliferation, lipopolysaccharide-induced B lymphocyte proliferation, and granulocytic phagocytosis were significantly lower, and absolute numbers of mature CD 21 B lymphocytes, natural killer cell activity and lysozyme concentration were significantly higher in dolphins with positive Chlamydiaceae antibody titers compared to seronegative healthy dolphins. Additionally, dolphins with positive Chlamydiaceae antibody titers had significant increases in ELISA antibody titers to Erysipelothrix rhusiopathiae. These data suggest that Chlamydiaceae infection may produce subclinical clinicoimmunopathologic perturbations that impact health. Any potential subclinical health impacts are important for the IRL and CHS dolphin populations, as past studies have indicated that both dolphin populations are affected by other complex infectious and neoplastic diseases, often associated with immunologic perturbations and anthropogenic contaminants.",
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