Clinical labeling and imaging of transplanted CD133+/CD34+ stem cells in patients with ischemic heart disease

Ali Ghodsizad, Viktor Bordel, Brian Bruckner, Matthias Loebe, Gunter Fuerst, I. Mirsaidi, M. Sucker, Arjang Ruhparwar, Matthias Karck, Hans Michael Klein

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The application of somatic stem cells has been shown to support the recovery of the myocardium in end-stage heart failure. A novel method for the intraoperative isolation and labeling of bone marrow-derived stem cells was established. After induction of general anesthesia, up to 400 mL of bone marrow were harvested from the posterior iliac crest and processed in the operating room under good manufacturing practice conditions by means of the automated cell-selection device Clini-MACS (Miltenyi Biotec). We subsequently injected autologous CD133+ and CD34+ stem cells in a predefined pattern around the laser channels in patients undergoing coronary artery bypass surgery and transmyocardial laser procedures. Intraoperative isolation and labeling is an effective cell-separation tool for the future, considering that novel cell markers can be promising new candidates for cell therapy.

Original languageEnglish (US)
JournalHeart Surgery Forum
Volume15
Issue number2
DOIs
StatePublished - Apr 2012
Externally publishedYes

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Myocardial Ischemia
Lasers
Stem Cells
Bone Marrow
Adult Stem Cells
Cell Separation
Operating Rooms
Cell- and Tissue-Based Therapy
Coronary Artery Bypass
General Anesthesia
Myocardium
Heart Failure
Equipment and Supplies

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Clinical labeling and imaging of transplanted CD133+/CD34+ stem cells in patients with ischemic heart disease. / Ghodsizad, Ali; Bordel, Viktor; Bruckner, Brian; Loebe, Matthias; Fuerst, Gunter; Mirsaidi, I.; Sucker, M.; Ruhparwar, Arjang; Karck, Matthias; Klein, Hans Michael.

In: Heart Surgery Forum, Vol. 15, No. 2, 04.2012.

Research output: Contribution to journalArticle

Ghodsizad, A, Bordel, V, Bruckner, B, Loebe, M, Fuerst, G, Mirsaidi, I, Sucker, M, Ruhparwar, A, Karck, M & Klein, HM 2012, 'Clinical labeling and imaging of transplanted CD133+/CD34+ stem cells in patients with ischemic heart disease', Heart Surgery Forum, vol. 15, no. 2. https://doi.org/10.1532/HSF98.20111138
Ghodsizad, Ali ; Bordel, Viktor ; Bruckner, Brian ; Loebe, Matthias ; Fuerst, Gunter ; Mirsaidi, I. ; Sucker, M. ; Ruhparwar, Arjang ; Karck, Matthias ; Klein, Hans Michael. / Clinical labeling and imaging of transplanted CD133+/CD34+ stem cells in patients with ischemic heart disease. In: Heart Surgery Forum. 2012 ; Vol. 15, No. 2.
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