Clathrin requirement for normal growth of yeast.

Sandra Lemmon, E. W. Jones

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Clathrin-coated membranes and coated vesicles take part in the selective transfer of proteins between different subcellular compartments of eukaryotic cells. To allow assessment of the role of clathrin in vesicular transport, genetic analysis of the clathrin heavy chain gene (CHC1) in Saccharomyces cerevisiae was initiated. The complete heavy chain gene was cloned, and the effects of deletion of this gene were studied. The null mutation (chc1-delta) is lethal unless a suppressor of clathrin deficiency (scd1) is present. Even in the presence of the suppressor gene, mutants lacking the clathrin heavy chain grow slowly, are genetically unstable, are morphologically abnormal, and show loss of or reduction in several yeast functions. These results indicate that clathrin is required for normal growth of yeast, and, therefore, most likely, for growth of all eukaryotic cells.

Original languageEnglish
Pages (from-to)504-509
Number of pages6
JournalScience
Volume238
Issue number4826
StatePublished - Oct 23 1987
Externally publishedYes

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Clathrin
Clathrin Heavy Chains
Yeasts
Eukaryotic Cells
Growth
Coated Vesicles
Suppressor Genes
Gene Deletion
Genes
Saccharomyces cerevisiae
Mutation
Membranes
Proteins

ASJC Scopus subject areas

  • General

Cite this

Lemmon, S., & Jones, E. W. (1987). Clathrin requirement for normal growth of yeast. Science, 238(4826), 504-509.

Clathrin requirement for normal growth of yeast. / Lemmon, Sandra; Jones, E. W.

In: Science, Vol. 238, No. 4826, 23.10.1987, p. 504-509.

Research output: Contribution to journalArticle

Lemmon, S & Jones, EW 1987, 'Clathrin requirement for normal growth of yeast.', Science, vol. 238, no. 4826, pp. 504-509.
Lemmon S, Jones EW. Clathrin requirement for normal growth of yeast. Science. 1987 Oct 23;238(4826):504-509.
Lemmon, Sandra ; Jones, E. W. / Clathrin requirement for normal growth of yeast. In: Science. 1987 ; Vol. 238, No. 4826. pp. 504-509.
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