Class-switch recombination in the absence of the igh 39 regulatory region

Ahrom Kim, Li Han, Gabriel E. Santiago, Ramiro E Verdun, Kefei Yu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The ∼28-kb 39 regulatory region (39RR), which is located at the most distal 39 region of the Ig H chain locus, has multiple regulatory functions that control IgH expression, class-switch recombination (CSR), and somatic hypermutation. In this article, we report that deletion of the entire 39RR in a mouse B cell line that is capable of robust cytokine-dependent CSR to IgA results in reduced, but not abolished, CSR. These data suggest that 39RR is not absolutely required for CSR and, thus, is not essential for targeting activation-induced cytidine deaminase to S regions, as was suggested. Moreover, replacing 39RR with a DNA fragment including only its four DNase I hypersensitive sites (lacking the large spacer regions) restores CSR to a level equivalent to or even higher than in wild-Type cells, suggesting that the four hypersensitive sites contain most of the CSR-promoting functions of 39RR. Stimulated cells express abundant germline transcripts, with the presence or absence of 39RR, providing evidence that 39RR has a role in promoting CSR that is unique from enhancing S region transcription. The Journal of Immunology, 2016, 197: 2930-2935.

Original languageEnglish (US)
Pages (from-to)2930-2935
Number of pages6
JournalJournal of Immunology
Volume197
Issue number7
DOIs
StatePublished - Oct 1 2016

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Nucleic Acid Regulatory Sequences
Genetic Recombination
Deoxyribonuclease I
Allergy and Immunology
Immunoglobulin A
B-Lymphocytes
Cytokines
Cell Line
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Class-switch recombination in the absence of the igh 39 regulatory region. / Kim, Ahrom; Han, Li; Santiago, Gabriel E.; Verdun, Ramiro E; Yu, Kefei.

In: Journal of Immunology, Vol. 197, No. 7, 01.10.2016, p. 2930-2935.

Research output: Contribution to journalArticle

Kim, Ahrom ; Han, Li ; Santiago, Gabriel E. ; Verdun, Ramiro E ; Yu, Kefei. / Class-switch recombination in the absence of the igh 39 regulatory region. In: Journal of Immunology. 2016 ; Vol. 197, No. 7. pp. 2930-2935.
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