Citron binds to PSD-95 at glutamatergic synapses on inhibitory neurons in the hippocampus

Wandong Zhang, Luis E Vazquez, Michelle Apperson, Mary B. Kennedy

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

Synaptic NMDA-type glutamate receptors are anchored to the second of three PDZ (PSD-95/Discs large/ZO-1) domains in the postsynaptic density (PSD) protein PSD-95. Here, we report that citron, a protein target for the activated form of the small GTP-binding protein Rho, preferentially binds the third PDZ domain of PSD-95. In GABAergic neurons from the hippocampus, citron forms a complex with PSD-95 and is concentrated at the postsynaptic side of glutamatergic synapses. Citron is expressed only at low levels in glutamatergic neurons in the hippocampus and is not detectable at synapses onto these neurons. In contrast to citron, p135 SynGAP, an abundant synaptic Ras GTPase-activating protein that can bind to all three PDZ domains of PSD- 95, and Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) are concentrated postsynaptically at glutamatergic synapses on glutamatergic neurons. CaM kinase II is not expressed and p135 SynGAP is expressed in less than half of hippocampal GABAergic neurons. Segregation of citron into inhibitory neurons does not occur in other brain regions. For example, citron is expressed at high levels in most thalamic neurons, which are primarily glutamatergic and contain CaM kinase II. In several other brain regions, citron is present in a subset of neurons that can be either GABAergic or glutamatergic and can sometimes express CaM kinase II. Thus, in the hippocampus, signal transduction complexes associated with postsynaptic NMDA receptors are different in glutamatergic and GABAergic neurons and are specialized in a way that is specific to the hippocampus.

Original languageEnglish (US)
Pages (from-to)96-108
Number of pages13
JournalJournal of Neuroscience
Volume19
Issue number1
StatePublished - Jan 1 1999
Externally publishedYes

Fingerprint

Post-Synaptic Density
Synapses
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Hippocampus
Neurons
GABAergic Neurons
N-Methyl-D-Aspartate Receptors
ras GTPase-Activating Proteins
rho GTP-Binding Proteins
Glutamate Receptors
Brain
Signal Transduction
Proteins

Keywords

  • Citron
  • Inhibitory neurons
  • Postsynaptic density
  • PSD-95
  • Signal transduction
  • Synaptic transmission

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Citron binds to PSD-95 at glutamatergic synapses on inhibitory neurons in the hippocampus. / Zhang, Wandong; Vazquez, Luis E; Apperson, Michelle; Kennedy, Mary B.

In: Journal of Neuroscience, Vol. 19, No. 1, 01.01.1999, p. 96-108.

Research output: Contribution to journalArticle

Zhang, Wandong ; Vazquez, Luis E ; Apperson, Michelle ; Kennedy, Mary B. / Citron binds to PSD-95 at glutamatergic synapses on inhibitory neurons in the hippocampus. In: Journal of Neuroscience. 1999 ; Vol. 19, No. 1. pp. 96-108.
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