CIS - Cloning of identical sequences between two complex genomes

Veronika Zabarovska, Jingfeng Li, Olga Muravenko, Ludmila Fedorova, Eleonora Braga, Ingemar Ernberg, Claes R Wahlestedt, George Klein, Eugene R. Zabarovsky

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Development of the methods permitting cloning of identical sequences between two sources of DNA can be very useful for many purposes, including isolation of disease genes. Here we describe a new method called CIS (cloning of identical sequences). A combination of digestion with MvnI, treatment with mung bean nuclease, UDG (uracil-DNA glycosylase) and PCR with 5'-methyl-dCTP and dUTP was used to isolate identical sequences between two micro-cell hybrid lines (MCH). In a control experiment, mouse MCH903.1 and MCH939.2 containing human chromosome 3 from different individuals, were compared using the CIS procedure. Only background fluorescence in-situ hybridization (FISH) was achieved. In another experiment, mouse MCH903.1, containing complete human chromosome 3, and rat MCH429.11, containing a part of human 3q from the same chromosome were compared. The experiment showed that the original MCH429.11 and the DNA purified using the CIS procedure had identical FISH patterns to human metaphase chromosomes, thus demonstrating the efficiency of CIS.

Original languageEnglish
Pages (from-to)77-84
Number of pages8
JournalChromosome Research
Volume8
Issue number1
DOIs
StatePublished - Mar 14 2000
Externally publishedYes

Fingerprint

Organism Cloning
Genome
Human Chromosomes
Chromosomes, Human, Pair 3
Fluorescence In Situ Hybridization
Uracil-DNA Glycosidase
DNA
Metaphase
Digestion
Chromosomes
Cell Line
Polymerase Chain Reaction
Genes
Therapeutics

Keywords

  • Chromosome mapping
  • Genetic technique
  • Human genetics
  • In-situ hybridization methods
  • Molecular cloning

ASJC Scopus subject areas

  • Genetics

Cite this

Zabarovska, V., Li, J., Muravenko, O., Fedorova, L., Braga, E., Ernberg, I., ... Zabarovsky, E. R. (2000). CIS - Cloning of identical sequences between two complex genomes. Chromosome Research, 8(1), 77-84. https://doi.org/10.1023/A:1009243606611

CIS - Cloning of identical sequences between two complex genomes. / Zabarovska, Veronika; Li, Jingfeng; Muravenko, Olga; Fedorova, Ludmila; Braga, Eleonora; Ernberg, Ingemar; Wahlestedt, Claes R; Klein, George; Zabarovsky, Eugene R.

In: Chromosome Research, Vol. 8, No. 1, 14.03.2000, p. 77-84.

Research output: Contribution to journalArticle

Zabarovska, V, Li, J, Muravenko, O, Fedorova, L, Braga, E, Ernberg, I, Wahlestedt, CR, Klein, G & Zabarovsky, ER 2000, 'CIS - Cloning of identical sequences between two complex genomes', Chromosome Research, vol. 8, no. 1, pp. 77-84. https://doi.org/10.1023/A:1009243606611
Zabarovska V, Li J, Muravenko O, Fedorova L, Braga E, Ernberg I et al. CIS - Cloning of identical sequences between two complex genomes. Chromosome Research. 2000 Mar 14;8(1):77-84. https://doi.org/10.1023/A:1009243606611
Zabarovska, Veronika ; Li, Jingfeng ; Muravenko, Olga ; Fedorova, Ludmila ; Braga, Eleonora ; Ernberg, Ingemar ; Wahlestedt, Claes R ; Klein, George ; Zabarovsky, Eugene R. / CIS - Cloning of identical sequences between two complex genomes. In: Chromosome Research. 2000 ; Vol. 8, No. 1. pp. 77-84.
@article{e96d7a8f4e80401786ac25c4a349e812,
title = "CIS - Cloning of identical sequences between two complex genomes",
abstract = "Development of the methods permitting cloning of identical sequences between two sources of DNA can be very useful for many purposes, including isolation of disease genes. Here we describe a new method called CIS (cloning of identical sequences). A combination of digestion with MvnI, treatment with mung bean nuclease, UDG (uracil-DNA glycosylase) and PCR with 5'-methyl-dCTP and dUTP was used to isolate identical sequences between two micro-cell hybrid lines (MCH). In a control experiment, mouse MCH903.1 and MCH939.2 containing human chromosome 3 from different individuals, were compared using the CIS procedure. Only background fluorescence in-situ hybridization (FISH) was achieved. In another experiment, mouse MCH903.1, containing complete human chromosome 3, and rat MCH429.11, containing a part of human 3q from the same chromosome were compared. The experiment showed that the original MCH429.11 and the DNA purified using the CIS procedure had identical FISH patterns to human metaphase chromosomes, thus demonstrating the efficiency of CIS.",
keywords = "Chromosome mapping, Genetic technique, Human genetics, In-situ hybridization methods, Molecular cloning",
author = "Veronika Zabarovska and Jingfeng Li and Olga Muravenko and Ludmila Fedorova and Eleonora Braga and Ingemar Ernberg and Wahlestedt, {Claes R} and George Klein and Zabarovsky, {Eugene R.}",
year = "2000",
month = "3",
day = "14",
doi = "10.1023/A:1009243606611",
language = "English",
volume = "8",
pages = "77--84",
journal = "Chromosome Research",
issn = "0967-3849",
publisher = "Springer Netherlands",
number = "1",

}

TY - JOUR

T1 - CIS - Cloning of identical sequences between two complex genomes

AU - Zabarovska, Veronika

AU - Li, Jingfeng

AU - Muravenko, Olga

AU - Fedorova, Ludmila

AU - Braga, Eleonora

AU - Ernberg, Ingemar

AU - Wahlestedt, Claes R

AU - Klein, George

AU - Zabarovsky, Eugene R.

PY - 2000/3/14

Y1 - 2000/3/14

N2 - Development of the methods permitting cloning of identical sequences between two sources of DNA can be very useful for many purposes, including isolation of disease genes. Here we describe a new method called CIS (cloning of identical sequences). A combination of digestion with MvnI, treatment with mung bean nuclease, UDG (uracil-DNA glycosylase) and PCR with 5'-methyl-dCTP and dUTP was used to isolate identical sequences between two micro-cell hybrid lines (MCH). In a control experiment, mouse MCH903.1 and MCH939.2 containing human chromosome 3 from different individuals, were compared using the CIS procedure. Only background fluorescence in-situ hybridization (FISH) was achieved. In another experiment, mouse MCH903.1, containing complete human chromosome 3, and rat MCH429.11, containing a part of human 3q from the same chromosome were compared. The experiment showed that the original MCH429.11 and the DNA purified using the CIS procedure had identical FISH patterns to human metaphase chromosomes, thus demonstrating the efficiency of CIS.

AB - Development of the methods permitting cloning of identical sequences between two sources of DNA can be very useful for many purposes, including isolation of disease genes. Here we describe a new method called CIS (cloning of identical sequences). A combination of digestion with MvnI, treatment with mung bean nuclease, UDG (uracil-DNA glycosylase) and PCR with 5'-methyl-dCTP and dUTP was used to isolate identical sequences between two micro-cell hybrid lines (MCH). In a control experiment, mouse MCH903.1 and MCH939.2 containing human chromosome 3 from different individuals, were compared using the CIS procedure. Only background fluorescence in-situ hybridization (FISH) was achieved. In another experiment, mouse MCH903.1, containing complete human chromosome 3, and rat MCH429.11, containing a part of human 3q from the same chromosome were compared. The experiment showed that the original MCH429.11 and the DNA purified using the CIS procedure had identical FISH patterns to human metaphase chromosomes, thus demonstrating the efficiency of CIS.

KW - Chromosome mapping

KW - Genetic technique

KW - Human genetics

KW - In-situ hybridization methods

KW - Molecular cloning

UR - http://www.scopus.com/inward/record.url?scp=0034011771&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034011771&partnerID=8YFLogxK

U2 - 10.1023/A:1009243606611

DO - 10.1023/A:1009243606611

M3 - Article

C2 - 10730592

AN - SCOPUS:0034011771

VL - 8

SP - 77

EP - 84

JO - Chromosome Research

JF - Chromosome Research

SN - 0967-3849

IS - 1

ER -