Circulating prosomatostatin-derived peptides. Differential responses to food ingestion

J. W. Ensinck, E. C. Laschansky, R. E. Vogel, D. A. Simonowitz, B. A. Roos, B. H. Francis

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Prosomatostatin (pro-S) and its bioactive posttranslational products, somatostatin-14 (S-14), somatostatin-13 (S-13), and somatostatin-28 (S-28), were measured in human plasma by the use of immunoglobulins to the NH2-terminus of S-28 conjugated with agarose to separate them and, thereafter, by RIA with an antiserum recognizing the COOH-terminus of pro-S, and by specific RIA for the NH2-terminus of S-14 and pro-S. In healthy men, mean basal levels of pro-S were 4 pg equivalent S-14/ml; S-14/S-13 combined were 9 pg equivalent S-14/ml; and S-28 levels were 16 pg/ml. After a 700-kcal meal, pro-S, S-14, and S-14/S-13 did not change, whereas S-28 levels doubled by 120 min and remained elevated for 240 min. To evaluate the origins of these peptides, their levels were compared in peripheral, portal, gastric, and mesenteric veins of anesthetized patients and in patients with total resection of stomach and pancreas before and after nutrient intake. The stomach and small intestine were sources of both peptides; however, most S-28 originated in the small intestine. These findings suggest that, in contrast to S-14, S-28 is a hormone and may modulate postprandial nutrient absorption and use.

Original languageEnglish
Pages (from-to)1580-1589
Number of pages10
JournalJournal of Clinical Investigation
Volume83
Issue number5
StatePublished - Jan 1 1989
Externally publishedYes

Fingerprint

5-chloro-3-tert-butyl-2'-chloro-4'-nitrosalicylanilide
Stomach
Eating
Somatostatin
Food
Peptides
Small Intestine
Somatostatin-28
Mesenteric Veins
Portal Vein
Sepharose
Meals
Immunoglobulins
Immune Sera
Pancreas
Hormones
prosomatostatin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Ensinck, J. W., Laschansky, E. C., Vogel, R. E., Simonowitz, D. A., Roos, B. A., & Francis, B. H. (1989). Circulating prosomatostatin-derived peptides. Differential responses to food ingestion. Journal of Clinical Investigation, 83(5), 1580-1589.

Circulating prosomatostatin-derived peptides. Differential responses to food ingestion. / Ensinck, J. W.; Laschansky, E. C.; Vogel, R. E.; Simonowitz, D. A.; Roos, B. A.; Francis, B. H.

In: Journal of Clinical Investigation, Vol. 83, No. 5, 01.01.1989, p. 1580-1589.

Research output: Contribution to journalArticle

Ensinck, JW, Laschansky, EC, Vogel, RE, Simonowitz, DA, Roos, BA & Francis, BH 1989, 'Circulating prosomatostatin-derived peptides. Differential responses to food ingestion', Journal of Clinical Investigation, vol. 83, no. 5, pp. 1580-1589.
Ensinck JW, Laschansky EC, Vogel RE, Simonowitz DA, Roos BA, Francis BH. Circulating prosomatostatin-derived peptides. Differential responses to food ingestion. Journal of Clinical Investigation. 1989 Jan 1;83(5):1580-1589.
Ensinck, J. W. ; Laschansky, E. C. ; Vogel, R. E. ; Simonowitz, D. A. ; Roos, B. A. ; Francis, B. H. / Circulating prosomatostatin-derived peptides. Differential responses to food ingestion. In: Journal of Clinical Investigation. 1989 ; Vol. 83, No. 5. pp. 1580-1589.
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