Circulating Progenitor Cells Can Be Reliably Identified on the Basis of Aldehyde Dehydrogenase Activity

Thomas J. Povsic, Katherine L. Zavodni, Francine L. Kelly, Shoukang Zhu, Pascal Goldschmidt-Clermont, Chunming Dong, Eric D. Peterson

Research output: Contribution to journalArticle

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Abstract

Objectives: Our objective was to develop and assess a novel endogenous progenitor cell (EPC) assay based on aldehyde dehydrogenase (ALDH) activity, and to define the relationship of ALDH-bright (ALDHbr) cells with previously defined EPCs, patient age, and extent of coronary artery disease. Background: Accurate assessment of circulating EPCs is of significant interest, yet current assays have limitations. Progenitor cells display high levels of ALDH activity. An assay based on ALDH activity may offer a simple means for enumerating EPCs. Methods: We simultaneously determined the numbers of EPCs based on ALDH activity and cell surface expression of CD133, CD34, and vascular endothelial growth factor receptor-2 in 110 patients undergoing cardiac catheterization. We assessed the reproducibility of these estimates, correlation among EPC assays, and the association of ALDHbr numbers with age and disease severity. Results: Aldehyde dehydrogenase-bright cells were easily identified in nonmobilized peripheral blood with median and mean frequencies of 0.041% and 0.074%, respectively. Aldehyde dehydrogenase-bright cells expressed CD34 or CD133 cell surface markers (57.0% and 27.1%, respectively), correlated closely with CD133+CD34+ cells (r = 0.72; p < 0.001), and differentiated into endothelial cells with greater efficiency than CD133+CD34+ cells. Aldehyde dehydrogenase-bright cell numbers were inversely associated with patient age and coronary disease severity. Conclusions: Aldehyde dehydrogenase activity represents a novel simplified method for quantifying EPCs. The correlation of ALDHbr cells with clinical factors and outcomes warrants further study.

Original languageEnglish
Pages (from-to)2243-2248
Number of pages6
JournalJournal of the American College of Cardiology
Volume50
Issue number23
DOIs
StatePublished - Dec 4 2007

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Aldehyde Dehydrogenase
Stem Cells
Vascular Endothelial Growth Factor Receptor-2
Cardiac Catheterization
Coronary Disease
Coronary Artery Disease
Endothelial Cells
Cell Count
erucylphosphocholine

ASJC Scopus subject areas

  • Nursing(all)

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Circulating Progenitor Cells Can Be Reliably Identified on the Basis of Aldehyde Dehydrogenase Activity. / Povsic, Thomas J.; Zavodni, Katherine L.; Kelly, Francine L.; Zhu, Shoukang; Goldschmidt-Clermont, Pascal; Dong, Chunming; Peterson, Eric D.

In: Journal of the American College of Cardiology, Vol. 50, No. 23, 04.12.2007, p. 2243-2248.

Research output: Contribution to journalArticle

Povsic, Thomas J. ; Zavodni, Katherine L. ; Kelly, Francine L. ; Zhu, Shoukang ; Goldschmidt-Clermont, Pascal ; Dong, Chunming ; Peterson, Eric D. / Circulating Progenitor Cells Can Be Reliably Identified on the Basis of Aldehyde Dehydrogenase Activity. In: Journal of the American College of Cardiology. 2007 ; Vol. 50, No. 23. pp. 2243-2248.
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abstract = "Objectives: Our objective was to develop and assess a novel endogenous progenitor cell (EPC) assay based on aldehyde dehydrogenase (ALDH) activity, and to define the relationship of ALDH-bright (ALDHbr) cells with previously defined EPCs, patient age, and extent of coronary artery disease. Background: Accurate assessment of circulating EPCs is of significant interest, yet current assays have limitations. Progenitor cells display high levels of ALDH activity. An assay based on ALDH activity may offer a simple means for enumerating EPCs. Methods: We simultaneously determined the numbers of EPCs based on ALDH activity and cell surface expression of CD133, CD34, and vascular endothelial growth factor receptor-2 in 110 patients undergoing cardiac catheterization. We assessed the reproducibility of these estimates, correlation among EPC assays, and the association of ALDHbr numbers with age and disease severity. Results: Aldehyde dehydrogenase-bright cells were easily identified in nonmobilized peripheral blood with median and mean frequencies of 0.041{\%} and 0.074{\%}, respectively. Aldehyde dehydrogenase-bright cells expressed CD34 or CD133 cell surface markers (57.0{\%} and 27.1{\%}, respectively), correlated closely with CD133+CD34+ cells (r = 0.72; p < 0.001), and differentiated into endothelial cells with greater efficiency than CD133+CD34+ cells. Aldehyde dehydrogenase-bright cell numbers were inversely associated with patient age and coronary disease severity. Conclusions: Aldehyde dehydrogenase activity represents a novel simplified method for quantifying EPCs. The correlation of ALDHbr cells with clinical factors and outcomes warrants further study.",
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AU - Zavodni, Katherine L.

AU - Kelly, Francine L.

AU - Zhu, Shoukang

AU - Goldschmidt-Clermont, Pascal

AU - Dong, Chunming

AU - Peterson, Eric D.

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