Circadian expression of genes involved in the purine biosynthetic pathway of the cyanobacterium Synechococcus sp. strain PCC 7942

Yi Liu, Nicholas Tsinoremas, Susan S. Golden, Takao Kondo, Carl Hirschie Johnson

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Extensive circadian (daily) control over gene expression in the cyanobacterium Synechococcus sp. strain PCC 7942 is programmed into at least two differentially phased groups. The transcriptional activity of the smaller group of genes is maximal at about dawn and minimal at about dusk. We identified one of the genes belonging to this latter group as purF, which encodes the key regulatory enzyme in the de novo purine synthetic pathway, glutamine PRPP amidotransferase (also known as amidophosphoribosyltransferase). Its expression pattern as a function of circadian time was confirmed by both luminescence from a purF::luxAB reporter strain and the abundance of purF mRNA. By fusing sequences upstream of the purF coding region to promoterless luxAB genes, we identified a limited upstream region, which potentially regulates purF circadian expression patterns in vivo. We also identified the purL gene immediately upstream of purF. The purL gene encodes FGAM synthetase, the fourth enzyme in the purine nucleotide biosynthesis pathway. Although these genes are expressed as part of a larger operon in other bacteria, reporter gene fusions revealed that purF and purL are transcribed independently in Synechococcus and that they are expressed at different phases of the circadian cycle. This differential expression pattern may be related to the oxygen sensitivity of amidophosphoribosyltransferase.

Original languageEnglish
Pages (from-to)1071-1081
Number of pages11
JournalMolecular Microbiology
Volume20
Issue number5
DOIs
StatePublished - Jul 9 1996
Externally publishedYes

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Synechococcus
Biosynthetic Pathways
Cyanobacteria
Gene Expression
Amidophosphoribosyltransferase
Genes
Purine Nucleotides
Gene Fusion
Enzymes
Operon
Luminescence
Glutamine
Reporter Genes
purine
Oxygen
Bacteria
Messenger RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

Circadian expression of genes involved in the purine biosynthetic pathway of the cyanobacterium Synechococcus sp. strain PCC 7942. / Liu, Yi; Tsinoremas, Nicholas; Golden, Susan S.; Kondo, Takao; Johnson, Carl Hirschie.

In: Molecular Microbiology, Vol. 20, No. 5, 09.07.1996, p. 1071-1081.

Research output: Contribution to journalArticle

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