Circadian clock mutants of cyanobacteria

Takao Kondo, Nicholas Tsinoremas, Susan S. Golden, Carl Hirschie Johnson, Shinsuke Kutsuna, Masahiro Ishiura

Research output: Contribution to journalArticle

232 Citations (Scopus)

Abstract

A diverse set of circadian clock mutants was isolated in a cyanobacterial strain that carries a bacterial luciferase reporter gene attached to a clock-controlled promoter. Among 150,000 clones of chemically mutagenized bioluminescent cells, 12 mutants were isolated that exhibit a broad spectrum of periods (between 16 and 60 hours), and 5 mutants were found that show a variety of unusual patterns, including arrhythmia. These mutations appear to be clock-specific. Moreover, it was demonstrated that in this cyanobacterium it is possible to clone mutant genes by complementation, which provides a means to genetically dissect the circadian mechanism.

Original languageEnglish
Pages (from-to)1233-1238
Number of pages6
JournalScience
Volume266
Issue number5188
StatePublished - Nov 18 1994
Externally publishedYes

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Circadian Clocks
Cyanobacteria
Bacterial Luciferases
Clone Cells
Reporter Genes
Cardiac Arrhythmias
Mutation
Genes

ASJC Scopus subject areas

  • General

Cite this

Kondo, T., Tsinoremas, N., Golden, S. S., Johnson, C. H., Kutsuna, S., & Ishiura, M. (1994). Circadian clock mutants of cyanobacteria. Science, 266(5188), 1233-1238.

Circadian clock mutants of cyanobacteria. / Kondo, Takao; Tsinoremas, Nicholas; Golden, Susan S.; Johnson, Carl Hirschie; Kutsuna, Shinsuke; Ishiura, Masahiro.

In: Science, Vol. 266, No. 5188, 18.11.1994, p. 1233-1238.

Research output: Contribution to journalArticle

Kondo, T, Tsinoremas, N, Golden, SS, Johnson, CH, Kutsuna, S & Ishiura, M 1994, 'Circadian clock mutants of cyanobacteria', Science, vol. 266, no. 5188, pp. 1233-1238.
Kondo T, Tsinoremas N, Golden SS, Johnson CH, Kutsuna S, Ishiura M. Circadian clock mutants of cyanobacteria. Science. 1994 Nov 18;266(5188):1233-1238.
Kondo, Takao ; Tsinoremas, Nicholas ; Golden, Susan S. ; Johnson, Carl Hirschie ; Kutsuna, Shinsuke ; Ishiura, Masahiro. / Circadian clock mutants of cyanobacteria. In: Science. 1994 ; Vol. 266, No. 5188. pp. 1233-1238.
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