CIP4 is required for the hypertrophic growth of neonatal cardiac myocytes

Francesca Rusconi, Hrishikesh Thakur, Jinliang Li, Michael S Kapiloff

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: CIP4 is a scaffold protein that regulates membrane deformation and tubulation, organization of the actin cytoskeleton, endocytosis of growth factor receptors, and vesicle trafficking. Although expressed in the heart, CIP4 has not been studied with regards to its potential function in cardiac myocytes. Results: We now show using RNA interference that CIP4 expression in neonatal rat ventricular myocytes is required for the induction of non-mitotic, hypertrophic growth by the -adrenergic agonist phenylephrine, the IL-6 cytokine leukemia inhibitor factor, and fetal bovine serum, as assayed using morphometry, immunocytochemistry for the hypertrophic marker atrial natriuretic factor and [§ssup§3§esup§H]leucine incorporation for de novo protein synthesis. This requirement was consistent with the induction of CIP4 expression by hypertrophic stimulation. The inhibition of myocyte hypertrophy by CIP4 small interfering oligonucleotides (siRNA) was rescued by expression of a recombinant CIP4 protein, but not by a mutant lacking the N-terminal FCH domain responsible for CIP4 intracellular localization. Conclusions: These results imply that CIP4 plays a significant role in the intracellular hypertrophic signal transduction network that controls the growth of cardiac myocytes in heart disease.

Original languageEnglish
Article number56
JournalJournal of Biomedical Science
Volume20
Issue number1
DOIs
StatePublished - Aug 6 2013

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Cardiac Myocytes
Muscle Cells
Recombinant proteins
Signal transduction
Adrenergic Agonists
Growth Factor Receptors
Atrial Natriuretic Factor
Phenylephrine
Growth
RNA Interference
Endocytosis
Actin Cytoskeleton
Recombinant Proteins
Scaffolds
Leucine
Oligonucleotides
Hypertrophy
Small Interfering RNA
Rats
Actins

Keywords

  • CIP4
  • Heart
  • Hypertrophy
  • Myocyte

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology
  • Biochemistry, medical
  • Endocrinology, Diabetes and Metabolism
  • Pharmacology (medical)

Cite this

CIP4 is required for the hypertrophic growth of neonatal cardiac myocytes. / Rusconi, Francesca; Thakur, Hrishikesh; Li, Jinliang; Kapiloff, Michael S.

In: Journal of Biomedical Science, Vol. 20, No. 1, 56, 06.08.2013.

Research output: Contribution to journalArticle

Rusconi, Francesca ; Thakur, Hrishikesh ; Li, Jinliang ; Kapiloff, Michael S. / CIP4 is required for the hypertrophic growth of neonatal cardiac myocytes. In: Journal of Biomedical Science. 2013 ; Vol. 20, No. 1.
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