Ciliary dysfunction impairs beta-cell insulin secretion and promotes development of type 2 diabetes in rodents

Jantje M. Gerdes; Sonia Christou-Savina; Yan Xiong; Tilo Moede; Noah Moruzzi; Patrick Karlsson-Edlund; Barbara Leibiger; Ingo B. Leibiger; Claes Göran Östenson; Philip L. Beales; Per Olof Berggren

doi:10.1038/ncomms6308

Ciliary dysfunction impairs beta-cell insulin secretion and promotes development of type 2 diabetes in rodents

Jantje M. Gerdes, Sonia Christou-Savina, Yan Xiong, Tilo Moede, Noah Moruzzi, Patrick Karlsson-Edlund, Barbara Leibiger, Ingo B. Leibiger, Claes Göran Östenson, Philip L. Beales, Per Olof Berggren

Surgery

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Type 2 diabetes mellitus is affecting more than 382 million people worldwide. Although much progress has been made, a comprehensive understanding of the underlying disease mechanism is still lacking. Here we report a role for the β-cell primary cilium in type 2 diabetes susceptibility. We find impaired glucose handling in young Bbs4^-/- mice before the onset of obesity. Basal body/ciliary perturbation in murine pancreatic islets leads to impaired first phase insulin release ex and in vivo. Insulin receptor is recruited to the cilium of stimulated β-cells and ciliary/basal body integrity is required for activation of downstream targets of insulin signalling. We also observe a reduction in the number of ciliated β-cells along with misregulated ciliary/basal body gene expression in pancreatic islets in a diabetic rat model. We suggest that ciliary function is implicated in insulin secretion and insulin signalling in the β-cell and that ciliary dysfunction could contribute to type 2 diabetes susceptibility.

Original language	English (US)
Article number	5308
Journal	Nature communications
Volume	5
DOIs	https://doi.org/10.1038/ncomms6308
State	Published - 2014

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/ncomms6308

Cite this

Ciliary dysfunction impairs beta-cell insulin secretion and promotes development of type 2 diabetes in rodents. / Gerdes, Jantje M.; Christou-Savina, Sonia; Xiong, Yan; Moede, Tilo; Moruzzi, Noah; Karlsson-Edlund, Patrick; Leibiger, Barbara; Leibiger, Ingo B.; Östenson, Claes Göran; Beales, Philip L.; Berggren, Per Olof.

In: Nature communications, Vol. 5, 5308, 2014.

Research output: Contribution to journal › Article › peer-review

Gerdes, Jantje M. ; Christou-Savina, Sonia ; Xiong, Yan ; Moede, Tilo ; Moruzzi, Noah ; Karlsson-Edlund, Patrick ; Leibiger, Barbara ; Leibiger, Ingo B. ; Östenson, Claes Göran ; Beales, Philip L. ; Berggren, Per Olof. / Ciliary dysfunction impairs beta-cell insulin secretion and promotes development of type 2 diabetes in rodents. In: Nature communications. 2014 ; Vol. 5.

@article{be22f051104b4270a881c43b1f459f07,

title = "Ciliary dysfunction impairs beta-cell insulin secretion and promotes development of type 2 diabetes in rodents",

abstract = "Type 2 diabetes mellitus is affecting more than 382 million people worldwide. Although much progress has been made, a comprehensive understanding of the underlying disease mechanism is still lacking. Here we report a role for the β-cell primary cilium in type 2 diabetes susceptibility. We find impaired glucose handling in young Bbs4-/- mice before the onset of obesity. Basal body/ciliary perturbation in murine pancreatic islets leads to impaired first phase insulin release ex and in vivo. Insulin receptor is recruited to the cilium of stimulated β-cells and ciliary/basal body integrity is required for activation of downstream targets of insulin signalling. We also observe a reduction in the number of ciliated β-cells along with misregulated ciliary/basal body gene expression in pancreatic islets in a diabetic rat model. We suggest that ciliary function is implicated in insulin secretion and insulin signalling in the β-cell and that ciliary dysfunction could contribute to type 2 diabetes susceptibility.",

author = "Gerdes, {Jantje M.} and Sonia Christou-Savina and Yan Xiong and Tilo Moede and Noah Moruzzi and Patrick Karlsson-Edlund and Barbara Leibiger and Leibiger, {Ingo B.} and {\"O}stenson, {Claes G{\"o}ran} and Beales, {Philip L.} and Berggren, {Per Olof}",

note = "Funding Information: We thank Elisabeth Nor{\'e}n-Krog, Elisabetta Dar{\'e}, Lisa Juntti-Berggren, Lars Selander, Alan Bahow, Gustavo de Abreu-Vieira, Hannelore Rother, Christopher Illies, as well as other members of the Berggren laboratory for their collegiality and helpful discussions. This work was supported by a Marie Curie International Re-Integration Grant (PIRG07-GA-2010-268397) and funds from F{\"o}reningen fr{\"a}mj{\"o}rande Diabetes Forskning (FFDF) and KI stiftelse to J.M.G., by funds from Karolinska Institutet and by grants from the Swedish Research Council, the Novo Nordisk Foundation, the Swedish Diabetes Association, the Family Knut and Alice Wallenberg Foundation, Diabetes Research and Wellness Foundation, Berth von Kantzow{\textquoteright}s Foundation, the Skandia Insurance Company, Ltd, the Strategic Research Programme in Diabetes at Karolinska Institutet, ERC-2013-AdG 338936-BetaImage, the Stichting af Jochnick Foundation and the Family Erling-Persson Foundation. Publisher Copyright: {\textcopyright} 2014 Macmillan Publishers Limited. All rights reserved.",

year = "2014",

doi = "10.1038/ncomms6308",

language = "English (US)",

volume = "5",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Ciliary dysfunction impairs beta-cell insulin secretion and promotes development of type 2 diabetes in rodents

AU - Gerdes, Jantje M.

AU - Christou-Savina, Sonia

AU - Xiong, Yan

AU - Moede, Tilo

AU - Moruzzi, Noah

AU - Karlsson-Edlund, Patrick

AU - Leibiger, Barbara

AU - Leibiger, Ingo B.

AU - Östenson, Claes Göran

AU - Beales, Philip L.

AU - Berggren, Per Olof

N1 - Funding Information: We thank Elisabeth Norén-Krog, Elisabetta Daré, Lisa Juntti-Berggren, Lars Selander, Alan Bahow, Gustavo de Abreu-Vieira, Hannelore Rother, Christopher Illies, as well as other members of the Berggren laboratory for their collegiality and helpful discussions. This work was supported by a Marie Curie International Re-Integration Grant (PIRG07-GA-2010-268397) and funds from Föreningen främjörande Diabetes Forskning (FFDF) and KI stiftelse to J.M.G., by funds from Karolinska Institutet and by grants from the Swedish Research Council, the Novo Nordisk Foundation, the Swedish Diabetes Association, the Family Knut and Alice Wallenberg Foundation, Diabetes Research and Wellness Foundation, Berth von Kantzow’s Foundation, the Skandia Insurance Company, Ltd, the Strategic Research Programme in Diabetes at Karolinska Institutet, ERC-2013-AdG 338936-BetaImage, the Stichting af Jochnick Foundation and the Family Erling-Persson Foundation. Publisher Copyright: © 2014 Macmillan Publishers Limited. All rights reserved.

PY - 2014

Y1 - 2014

N2 - Type 2 diabetes mellitus is affecting more than 382 million people worldwide. Although much progress has been made, a comprehensive understanding of the underlying disease mechanism is still lacking. Here we report a role for the β-cell primary cilium in type 2 diabetes susceptibility. We find impaired glucose handling in young Bbs4-/- mice before the onset of obesity. Basal body/ciliary perturbation in murine pancreatic islets leads to impaired first phase insulin release ex and in vivo. Insulin receptor is recruited to the cilium of stimulated β-cells and ciliary/basal body integrity is required for activation of downstream targets of insulin signalling. We also observe a reduction in the number of ciliated β-cells along with misregulated ciliary/basal body gene expression in pancreatic islets in a diabetic rat model. We suggest that ciliary function is implicated in insulin secretion and insulin signalling in the β-cell and that ciliary dysfunction could contribute to type 2 diabetes susceptibility.

AB - Type 2 diabetes mellitus is affecting more than 382 million people worldwide. Although much progress has been made, a comprehensive understanding of the underlying disease mechanism is still lacking. Here we report a role for the β-cell primary cilium in type 2 diabetes susceptibility. We find impaired glucose handling in young Bbs4-/- mice before the onset of obesity. Basal body/ciliary perturbation in murine pancreatic islets leads to impaired first phase insulin release ex and in vivo. Insulin receptor is recruited to the cilium of stimulated β-cells and ciliary/basal body integrity is required for activation of downstream targets of insulin signalling. We also observe a reduction in the number of ciliated β-cells along with misregulated ciliary/basal body gene expression in pancreatic islets in a diabetic rat model. We suggest that ciliary function is implicated in insulin secretion and insulin signalling in the β-cell and that ciliary dysfunction could contribute to type 2 diabetes susceptibility.

UR - http://www.scopus.com/inward/record.url?scp=84923277743&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84923277743&partnerID=8YFLogxK

U2 - 10.1038/ncomms6308

DO - 10.1038/ncomms6308

M3 - Article

AN - SCOPUS:84923277743

VL - 5

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 5308

ER -

Ciliary dysfunction impairs beta-cell insulin secretion and promotes development of type 2 diabetes in rodents

Abstract

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this