CIITA variation in the presence of HLA-DRB1*1501 increases risk for multiple sclerosis

Paola G. Bronson, Stacy Caillier, Patricia P. Ramsay, Jacob L McCauley, Rebecca L. Zuvich, Philip L. De Jager, John D. Rioux, Adrian J. Ivinson, Alastair Compston, David A. Hafler, Stephen J. Sawcer, Margaret A Pericak-Vance, Jonathan L. Haines, Stephen L. Hauser, Jorge R. Oksenberg, Lisa F. Barcellos

Research output: Contribution to journalArticle

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Abstract

The MHC class II transactivator gene (CIITA) is an important transcription factor regulating gene required for HLA class II MHC-restricted antigen presentation. Association with HLA class II variation, particularly HLA-DRB1*1501, has been well-established for multiple sclerosis (MS). In addition, the -168A/G CIITA promoter variant (rs3087456) has been reported to be associated with MS. Thus, a multi-stage investigation of variation within CIITA, DRB1*1501 and MS was undertaken in 6108 individuals. In stage 1,24 SNPs within CIITA were genotyped in 1320 cases and 1363 controls (n = 2683). Rs4774 (missense -1614G/C; G500A) was associated with MS (P = 4.9 3 10-3), particularly in DRB1*1501 1 individuals (P = 1 × 10-4). No association was observed for the 2168A/G promoter variant. In stage 2, rs4774 was genotyped in 973 extended families; rs4774*C was also associated with increased risk for MS in DRB1*15011 families (P = 2.3 × 10-2). In a third analysis, rs4774 was tested in cases and controls (stage 1) combined with one case per family (stage 2) for increased power. Rs4774*C was associated with MS (P = 1 × 10-3), particularly in DRB1*15011 cases and controls (P = 1 3 10-4). Results obtained from logistic regression analysis showed evidence for interaction between rs4774*C and DRB1*1501 associated with risk for MS (ratio of ORs 5 1.72, 95% CI 1.28-2.32, P = 3 × 10-4). Furthermore, rs4774*C was associated with DRB1*15011 MS when conditioned on the presence (OR = 1.67, 95% CI = 1.19-2.37, P = 1.9 × 10-3) and absence (OR = 1.49, 95% CI = 1.15-1.95, P = 2.3 × 10-3) of CLEC16A rs6498169*G, a putative MS risk allele adjacent to CIITA. Our results provide strong evidence supporting a role for CIITA variation in MS risk, which appears to depend on the presence of DRB1*1501.

Original languageEnglish
Article numberddq101
Pages (from-to)2331-2340
Number of pages10
JournalHuman Molecular Genetics
Volume19
Issue number11
DOIs
StatePublished - Mar 8 2010

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HLA-DRB1 Chains
MHC Class II Genes
Multiple Sclerosis
MHC class II transactivator protein
Antigen Presentation
Single Nucleotide Polymorphism
Transcription Factors
Logistic Models
Alleles
Regression Analysis

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology

Cite this

Bronson, P. G., Caillier, S., Ramsay, P. P., McCauley, J. L., Zuvich, R. L., De Jager, P. L., ... Barcellos, L. F. (2010). CIITA variation in the presence of HLA-DRB1*1501 increases risk for multiple sclerosis. Human Molecular Genetics, 19(11), 2331-2340. [ddq101]. https://doi.org/10.1093/hmg/ddq101

CIITA variation in the presence of HLA-DRB1*1501 increases risk for multiple sclerosis. / Bronson, Paola G.; Caillier, Stacy; Ramsay, Patricia P.; McCauley, Jacob L; Zuvich, Rebecca L.; De Jager, Philip L.; Rioux, John D.; Ivinson, Adrian J.; Compston, Alastair; Hafler, David A.; Sawcer, Stephen J.; Pericak-Vance, Margaret A; Haines, Jonathan L.; Hauser, Stephen L.; Oksenberg, Jorge R.; Barcellos, Lisa F.

In: Human Molecular Genetics, Vol. 19, No. 11, ddq101, 08.03.2010, p. 2331-2340.

Research output: Contribution to journalArticle

Bronson, PG, Caillier, S, Ramsay, PP, McCauley, JL, Zuvich, RL, De Jager, PL, Rioux, JD, Ivinson, AJ, Compston, A, Hafler, DA, Sawcer, SJ, Pericak-Vance, MA, Haines, JL, Hauser, SL, Oksenberg, JR & Barcellos, LF 2010, 'CIITA variation in the presence of HLA-DRB1*1501 increases risk for multiple sclerosis', Human Molecular Genetics, vol. 19, no. 11, ddq101, pp. 2331-2340. https://doi.org/10.1093/hmg/ddq101
Bronson PG, Caillier S, Ramsay PP, McCauley JL, Zuvich RL, De Jager PL et al. CIITA variation in the presence of HLA-DRB1*1501 increases risk for multiple sclerosis. Human Molecular Genetics. 2010 Mar 8;19(11):2331-2340. ddq101. https://doi.org/10.1093/hmg/ddq101
Bronson, Paola G. ; Caillier, Stacy ; Ramsay, Patricia P. ; McCauley, Jacob L ; Zuvich, Rebecca L. ; De Jager, Philip L. ; Rioux, John D. ; Ivinson, Adrian J. ; Compston, Alastair ; Hafler, David A. ; Sawcer, Stephen J. ; Pericak-Vance, Margaret A ; Haines, Jonathan L. ; Hauser, Stephen L. ; Oksenberg, Jorge R. ; Barcellos, Lisa F. / CIITA variation in the presence of HLA-DRB1*1501 increases risk for multiple sclerosis. In: Human Molecular Genetics. 2010 ; Vol. 19, No. 11. pp. 2331-2340.
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abstract = "The MHC class II transactivator gene (CIITA) is an important transcription factor regulating gene required for HLA class II MHC-restricted antigen presentation. Association with HLA class II variation, particularly HLA-DRB1*1501, has been well-established for multiple sclerosis (MS). In addition, the -168A/G CIITA promoter variant (rs3087456) has been reported to be associated with MS. Thus, a multi-stage investigation of variation within CIITA, DRB1*1501 and MS was undertaken in 6108 individuals. In stage 1,24 SNPs within CIITA were genotyped in 1320 cases and 1363 controls (n = 2683). Rs4774 (missense -1614G/C; G500A) was associated with MS (P = 4.9 3 10-3), particularly in DRB1*1501 1 individuals (P = 1 × 10-4). No association was observed for the 2168A/G promoter variant. In stage 2, rs4774 was genotyped in 973 extended families; rs4774*C was also associated with increased risk for MS in DRB1*15011 families (P = 2.3 × 10-2). In a third analysis, rs4774 was tested in cases and controls (stage 1) combined with one case per family (stage 2) for increased power. Rs4774*C was associated with MS (P = 1 × 10-3), particularly in DRB1*15011 cases and controls (P = 1 3 10-4). Results obtained from logistic regression analysis showed evidence for interaction between rs4774*C and DRB1*1501 associated with risk for MS (ratio of ORs 5 1.72, 95{\%} CI 1.28-2.32, P = 3 × 10-4). Furthermore, rs4774*C was associated with DRB1*15011 MS when conditioned on the presence (OR = 1.67, 95{\%} CI = 1.19-2.37, P = 1.9 × 10-3) and absence (OR = 1.49, 95{\%} CI = 1.15-1.95, P = 2.3 × 10-3) of CLEC16A rs6498169*G, a putative MS risk allele adjacent to CIITA. Our results provide strong evidence supporting a role for CIITA variation in MS risk, which appears to depend on the presence of DRB1*1501.",
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AU - Bronson, Paola G.

AU - Caillier, Stacy

AU - Ramsay, Patricia P.

AU - McCauley, Jacob L

AU - Zuvich, Rebecca L.

AU - De Jager, Philip L.

AU - Rioux, John D.

AU - Ivinson, Adrian J.

AU - Compston, Alastair

AU - Hafler, David A.

AU - Sawcer, Stephen J.

AU - Pericak-Vance, Margaret A

AU - Haines, Jonathan L.

AU - Hauser, Stephen L.

AU - Oksenberg, Jorge R.

AU - Barcellos, Lisa F.

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N2 - The MHC class II transactivator gene (CIITA) is an important transcription factor regulating gene required for HLA class II MHC-restricted antigen presentation. Association with HLA class II variation, particularly HLA-DRB1*1501, has been well-established for multiple sclerosis (MS). In addition, the -168A/G CIITA promoter variant (rs3087456) has been reported to be associated with MS. Thus, a multi-stage investigation of variation within CIITA, DRB1*1501 and MS was undertaken in 6108 individuals. In stage 1,24 SNPs within CIITA were genotyped in 1320 cases and 1363 controls (n = 2683). Rs4774 (missense -1614G/C; G500A) was associated with MS (P = 4.9 3 10-3), particularly in DRB1*1501 1 individuals (P = 1 × 10-4). No association was observed for the 2168A/G promoter variant. In stage 2, rs4774 was genotyped in 973 extended families; rs4774*C was also associated with increased risk for MS in DRB1*15011 families (P = 2.3 × 10-2). In a third analysis, rs4774 was tested in cases and controls (stage 1) combined with one case per family (stage 2) for increased power. Rs4774*C was associated with MS (P = 1 × 10-3), particularly in DRB1*15011 cases and controls (P = 1 3 10-4). Results obtained from logistic regression analysis showed evidence for interaction between rs4774*C and DRB1*1501 associated with risk for MS (ratio of ORs 5 1.72, 95% CI 1.28-2.32, P = 3 × 10-4). Furthermore, rs4774*C was associated with DRB1*15011 MS when conditioned on the presence (OR = 1.67, 95% CI = 1.19-2.37, P = 1.9 × 10-3) and absence (OR = 1.49, 95% CI = 1.15-1.95, P = 2.3 × 10-3) of CLEC16A rs6498169*G, a putative MS risk allele adjacent to CIITA. Our results provide strong evidence supporting a role for CIITA variation in MS risk, which appears to depend on the presence of DRB1*1501.

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