Chronic prenatal exposure to cocaine alters cerebrovascular responses in newborn pigs

Massroor Pourcyrous, Henrietta S. Bada, Kari E. Blaho, Mildred M. Randolph, Helena Parfenova, Timothy D. Mandrell, Kristopher Arheart, Sheldon B. Korones, Charles W. Leffler

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Maternal cocaine abuse may increase the incidence of perinatal asphyxia. In nonexposed asphyxiated neonates, decreased cerebrospinal fluid (CSF) cAMP concentrations are associated with poor neurological outcome. On the other hand, cocaine increases central nervous system (CNS) cAMP. Therefore, we hypothesized that in utero cocaine exposure may increase brain cAMP and thereby preserve cerebrovascular responses to cAMP-dependent stimuli following asphyxia. Pregnant pigs received either cocaine (1 mg/kg, iv) twice weekly during the last trimester or normal saline vehicle (sham-control) and were allowed to deliver vaginally at term. Cranial windows were implanted in the newborn pigs within the first week of life and used to collect CSF for cAMP determinations and to assess changes in pial arteriolar diameters (PAD). In the first part of the study, pial arteriolar responses to different vasodilator and vasoconstrictor stimuli were evaluated in piglets prior to asphyxia (n = 20). In newborn pigs exposed to cocaine, cerebrovascular responses to hypercapnia and norepinephrine were significantly exaggerated compared to controls. Then, piglets were randomly selected for the second part of the study that involved prolonged asphyxia (n = 12). In cocaine-exposed but not sham-control piglets, CSF cAMP increased markedly during asphyxia. In the sham piglets, but not the cocaine-exposed piglets, CSF cAMP fell progressively below the baseline during recovery. Cerebrovascular reactivity to cAMP-dependent stimuli (hypercapnia and isoproterenol) was preserved during recovery from asphyxia in the cocaine-exposed piglets but significantly attenuated in the sham controls. We conclude that piglets with chronic prenatal exposure to cocaine show exaggerated cerebrovascular responses to vasogenic stimuli and preserved cAMP-dependant cerebral vasoreactivity following asphyxia.

Original languageEnglish
Pages (from-to)819-825
Number of pages7
JournalExperimental Biology and Medicine
Volume229
Issue number8
StatePublished - Sep 1 2004

Fingerprint

Cocaine
Asphyxia
Swine
Cerebrospinal fluid
Cerebrospinal Fluid
Hypercapnia
Cocaine-Related Disorders
Recovery
Third Pregnancy Trimester
Vasoconstrictor Agents
Vasodilator Agents
Isoproterenol
Neurology
Norepinephrine
Central Nervous System
Brain
Mothers
Incidence

Keywords

  • Asphyxia
  • cAMP
  • Cocaine
  • Neonates
  • Pial arterioles

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Pourcyrous, M., Bada, H. S., Blaho, K. E., Randolph, M. M., Parfenova, H., Mandrell, T. D., ... Leffler, C. W. (2004). Chronic prenatal exposure to cocaine alters cerebrovascular responses in newborn pigs. Experimental Biology and Medicine, 229(8), 819-825.

Chronic prenatal exposure to cocaine alters cerebrovascular responses in newborn pigs. / Pourcyrous, Massroor; Bada, Henrietta S.; Blaho, Kari E.; Randolph, Mildred M.; Parfenova, Helena; Mandrell, Timothy D.; Arheart, Kristopher; Korones, Sheldon B.; Leffler, Charles W.

In: Experimental Biology and Medicine, Vol. 229, No. 8, 01.09.2004, p. 819-825.

Research output: Contribution to journalArticle

Pourcyrous, M, Bada, HS, Blaho, KE, Randolph, MM, Parfenova, H, Mandrell, TD, Arheart, K, Korones, SB & Leffler, CW 2004, 'Chronic prenatal exposure to cocaine alters cerebrovascular responses in newborn pigs', Experimental Biology and Medicine, vol. 229, no. 8, pp. 819-825.
Pourcyrous M, Bada HS, Blaho KE, Randolph MM, Parfenova H, Mandrell TD et al. Chronic prenatal exposure to cocaine alters cerebrovascular responses in newborn pigs. Experimental Biology and Medicine. 2004 Sep 1;229(8):819-825.
Pourcyrous, Massroor ; Bada, Henrietta S. ; Blaho, Kari E. ; Randolph, Mildred M. ; Parfenova, Helena ; Mandrell, Timothy D. ; Arheart, Kristopher ; Korones, Sheldon B. ; Leffler, Charles W. / Chronic prenatal exposure to cocaine alters cerebrovascular responses in newborn pigs. In: Experimental Biology and Medicine. 2004 ; Vol. 229, No. 8. pp. 819-825.
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