Chronic fatigue syndrome is associated with diminished intracellular perforin

K. J. Maher, N. G. Klimas, Mary Ann Fletcher

Research output: Contribution to journalArticlepeer-review

82 Scopus citations

Abstract

Chronic fatigue syndrome (CFS) is an illness characterized by unexplained and prolonged fatigue that is often accompanied by abnormalities of immune, endocrine and cognitive functions. Diminished natural killer cell cytotoxicity (NKCC) is a frequently reported finding. However, the molecular basis of this defect of in vitro cytotoxicy has not been described. Perform is a protein found within intracellular granules of NK and cytotoxic T cells and is a key factor in the lytic processes mediated by these cells. Quantitative fluorescence flow cytometry was used to the intracellular perforin content in CFS subjects and healthy controls. A significant reduction in the NK cell associated perforin levels in samples from CFS patients, compared to healthy controls, was observed. There was also an indication of a reduced perforin level within the cytotoxic T cells of CFS subjects, providing the first evidence, to our knowledge, to suggest a T cell associated cytotoxic deficit in CFS. Because perforin is important in immune surveillance and homeostasis of the immune system, its deficiency may prove to be an important factor in the pathogenesis of CFS and its analysis may prove useful as a biomarker in the study of CFS.

Original languageEnglish (US)
Pages (from-to)505-511
Number of pages7
JournalClinical and Experimental Immunology
Volume142
Issue number3
DOIs
StatePublished - Dec 2005

Keywords

  • Chronic fatigue syndrome
  • Cytoplasmic granules
  • Flow cytometry
  • Killer cells - natural
  • Perforin

ASJC Scopus subject areas

  • Immunology

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