Chronic electronic cigarette exposure in mice induces features of COPD in a nicotine-dependent manner

Itsaso Garcia-Arcos, Patrick Geraghty, Nathalie Baumlin, Michael Campos, Abdoulaye Jules Dabo, Bakr Jundi, Neville Cummins, Edward Eden, Astrid Grosche, Matthias Salathe, Robert Foronjy

Research output: Contribution to journalArticle

97 Scopus citations

Abstract

Background: The use of electronic (e)-cigarettes is increasing rapidly, but their lung health effects are not established. Clinical studies examining the potential long-term impact of e-cigarette use on lung health will take decades. To address this gap in knowledge, this study investigated the effects of exposure to aerosolised nicotine-free and nicotine-containing e-cigarette fluid on mouse lungs and normal human airway epithelial cells. Methods: Mice were exposed to aerosolised phosphate-buffered saline, nicotine-free or nicotine-containing e-cigarette solution, 1-hour daily for 4 months. Normal human bronchial epithelial (NHBE) cells cultured at an air-liquid interface were exposed to e-cigarette vapours or nicotine solutions using a Vitrocell smoke exposure robot. Results: Inhalation of nicotine-containing e-cigarettes increased airway hyper-reactivity, distal airspace enlargement, mucin production, cytokine and protease expression. Exposure to nicotine-free e-cigarettes did not affect these lung parameters. NHBE cells exposed to nicotine-containing e-cigarette vapour showed impaired ciliary beat frequency, airway surface liquid volume, cystic fibrosis transmembrane regulator and ATP-stimulated K+ ion conductance and decreased expression of FOXJ1 and KCNMA1. Exposure of NHBE cells to nicotine for 5 days increased interleukin (IL)-6 and IL-8 secretion. Conclusions: Exposure to inhaled nicotine-containing e-cigarette fluids triggered effects normally associated with the development of COPD including cytokine expression, airway hyper-reactivity and lung tissue destruction. These effects were nicotine-dependent both in the mouse lung and in human airway cells, suggesting that inhaled nicotine contributes to airway and lung disease in addition to its addictive properties. Thus, these findings highlight the potential dangers of nicotine inhalation during e-cigarette use.

Original languageEnglish (US)
Pages (from-to)1119-1129
Number of pages11
JournalThorax
Volume71
Issue number12
DOIs
StatePublished - Dec 1 2016

Keywords

  • COPD Pathology
  • COPD ÀÜ Mechanisms
  • Emphysema

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

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    Garcia-Arcos, I., Geraghty, P., Baumlin, N., Campos, M., Dabo, A. J., Jundi, B., Cummins, N., Eden, E., Grosche, A., Salathe, M., & Foronjy, R. (2016). Chronic electronic cigarette exposure in mice induces features of COPD in a nicotine-dependent manner. Thorax, 71(12), 1119-1129. https://doi.org/10.1136/thoraxjnl-2015-208039