Chronic electronic cigarette exposure in mice induces features of COPD in a nicotine-dependent manner

Itsaso Garcia-Arcos, Patrick Geraghty, Nathalie Baumlin, Michael A Campos, Abdoulaye Jules Dabo, Bakr Jundi, Neville Cummins, Edward Eden, Astrid Grosche, Matthias A Salathe, Robert Foronjy

Research output: Contribution to journalArticle

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Abstract

Background: The use of electronic (e)-cigarettes is increasing rapidly, but their lung health effects are not established. Clinical studies examining the potential long-term impact of e-cigarette use on lung health will take decades. To address this gap in knowledge, this study investigated the effects of exposure to aerosolised nicotine-free and nicotine-containing e-cigarette fluid on mouse lungs and normal human airway epithelial cells. Methods: Mice were exposed to aerosolised phosphate-buffered saline, nicotine-free or nicotine-containing e-cigarette solution, 1-hour daily for 4 months. Normal human bronchial epithelial (NHBE) cells cultured at an air-liquid interface were exposed to e-cigarette vapours or nicotine solutions using a Vitrocell smoke exposure robot. Results: Inhalation of nicotine-containing e-cigarettes increased airway hyper-reactivity, distal airspace enlargement, mucin production, cytokine and protease expression. Exposure to nicotine-free e-cigarettes did not affect these lung parameters. NHBE cells exposed to nicotine-containing e-cigarette vapour showed impaired ciliary beat frequency, airway surface liquid volume, cystic fibrosis transmembrane regulator and ATP-stimulated K+ ion conductance and decreased expression of FOXJ1 and KCNMA1. Exposure of NHBE cells to nicotine for 5 days increased interleukin (IL)-6 and IL-8 secretion. Conclusions: Exposure to inhaled nicotine-containing e-cigarette fluids triggered effects normally associated with the development of COPD including cytokine expression, airway hyper-reactivity and lung tissue destruction. These effects were nicotine-dependent both in the mouse lung and in human airway cells, suggesting that inhaled nicotine contributes to airway and lung disease in addition to its addictive properties. Thus, these findings highlight the potential dangers of nicotine inhalation during e-cigarette use.

Original languageEnglish (US)
Pages (from-to)1119-1129
Number of pages11
JournalThorax
Volume71
Issue number12
DOIs
StatePublished - Dec 1 2016

Fingerprint

Nicotine
Chronic Obstructive Pulmonary Disease
Tobacco Products
Lung
Epithelial Cells
Electronic Cigarettes
Inhalation
Cytokines
Health
Mucins
Interleukin-8
Cystic Fibrosis
Smoke
Lung Diseases
Interleukin-6
Peptide Hydrolases
Adenosine Triphosphate
Phosphates
Air
Ions

Keywords

  • COPD Pathology
  • COPD ÀÜ Mechanisms
  • Emphysema

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Garcia-Arcos, I., Geraghty, P., Baumlin, N., Campos, M. A., Dabo, A. J., Jundi, B., ... Foronjy, R. (2016). Chronic electronic cigarette exposure in mice induces features of COPD in a nicotine-dependent manner. Thorax, 71(12), 1119-1129. https://doi.org/10.1136/thoraxjnl-2015-208039

Chronic electronic cigarette exposure in mice induces features of COPD in a nicotine-dependent manner. / Garcia-Arcos, Itsaso; Geraghty, Patrick; Baumlin, Nathalie; Campos, Michael A; Dabo, Abdoulaye Jules; Jundi, Bakr; Cummins, Neville; Eden, Edward; Grosche, Astrid; Salathe, Matthias A; Foronjy, Robert.

In: Thorax, Vol. 71, No. 12, 01.12.2016, p. 1119-1129.

Research output: Contribution to journalArticle

Garcia-Arcos, I, Geraghty, P, Baumlin, N, Campos, MA, Dabo, AJ, Jundi, B, Cummins, N, Eden, E, Grosche, A, Salathe, MA & Foronjy, R 2016, 'Chronic electronic cigarette exposure in mice induces features of COPD in a nicotine-dependent manner', Thorax, vol. 71, no. 12, pp. 1119-1129. https://doi.org/10.1136/thoraxjnl-2015-208039
Garcia-Arcos, Itsaso ; Geraghty, Patrick ; Baumlin, Nathalie ; Campos, Michael A ; Dabo, Abdoulaye Jules ; Jundi, Bakr ; Cummins, Neville ; Eden, Edward ; Grosche, Astrid ; Salathe, Matthias A ; Foronjy, Robert. / Chronic electronic cigarette exposure in mice induces features of COPD in a nicotine-dependent manner. In: Thorax. 2016 ; Vol. 71, No. 12. pp. 1119-1129.
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AU - Dabo, Abdoulaye Jules

AU - Jundi, Bakr

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